Document Detail

Acute and chronic effects of dietary fatty acids on cholecystokinin expression, storage and secretion in enteroendocrine STC-1 cells.
MedLine Citation:
PMID:  20352619     Owner:  NLM     Status:  MEDLINE    
Cholecystokinin (CCK) is a peptide hormone secreted from the I-cells of the intestine and it has important physiological actions related to appetite regulation and satiety. In this study we used STC-1 cells to investigate the effects of common dietary-derived fatty acids (FAs) on I-cell secretory function and metabolism. We extend earlier studies by measuring the acute and chronic effects of 11 FAs on CCK secretion, cellular CCK content, CCK mRNA levels, cellular DNA synthesis, cellular viability and cytotoxicity. FAs were selected in order to assess the importance of chain length, degree of saturation, and double bond position and conformation. The results demonstrate that secretory responses elicited by dietary FAs are highly selective. For example, altering the conformation of a double bond from cis to trans (i.e. oleic acid versus elaidic acid) completely abolishes CCK secretion. Lauric acid appears to adversely affect I-cell metabolism and arachidonic acid suppresses DNA synthesis. Our studies reveal for the first time that conjugated linoleic acid isoforms are particularly potent CCK secretagogues, which also boost intracellular stores of CCK. These actions of conjugated linoleic acid may explain satiating actions observed in dietary intervention studies.
Katharine V Hand; Christine M Bruen; Fiona O'Halloran; Linda Giblin; Brian D Green
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular nutrition & food research     Volume:  54 Suppl 1     ISSN:  1613-4133     ISO Abbreviation:  Mol Nutr Food Res     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-10     Completed Date:  2010-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101231818     Medline TA:  Mol Nutr Food Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  S93-S103     Citation Subset:  IM    
Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University Belfast, Belfast, UK.
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MeSH Terms
Cell Line, Tumor
Cell Survival / drug effects
Cholecystokinin / drug effects,  genetics*,  secretion
DNA Primers
DNA Replication / drug effects
Dietary Fats / pharmacology*
Fatty Acids / pharmacology
Gene Expression Regulation, Neoplastic
Mice, Transgenic
RNA, Messenger / genetics
RNA, Neoplasm / genetics
Reg. No./Substance:
0/DNA Primers; 0/Dietary Fats; 0/Fatty Acids; 0/RNA, Messenger; 0/RNA, Neoplasm; 9011-97-6/Cholecystokinin

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