Document Detail

Acute central infusion of leptin modulates fatty acid mobilization by affecting lipolysis and mRNA expression for uncoupling proteins.
MedLine Citation:
PMID:  15734723     Owner:  NLM     Status:  MEDLINE    
Chronic administration of leptin has been shown to reduce adiposity through energy intake and expenditure. The present study aims to examine how acute central infusion of leptin regulates peripheral lipid metabolism, as assessed by markers indicative of their mobilization and utilization. A bolus infusion of 1 microg/rat leptin into the third cerebroventricle increased the expression of mRNA for hormone-sensitive lipase (HSL), an indicator of lipolysis, in white adipose tissue (WAT). This was accompanied by elevation of plasma levels of glycerol, but not of free fatty acids, as compared to the saline control (P < 0.03). The same treatment with leptin decreased plasma insulin levels but did not affect the plasma glucose level (P < 0.05 for insulin). Among the major regulators of the transportation or utilization of energy substrates, leptin treatment increased expression of mRNA for uncoupling protein 1 (UCP1) in brown adipose tissue (BAT), UCP2 in WAT, and UCP3 in quadriceps skeletal muscle, but not those for fatty acid-binding protein in WAT, carnitine phosphate transferase-1, a marker for beta oxidation of fatty acids in muscle, nor glucose transporter 4 in WAT and muscle (P < 0.01 for HSL, P < 0.05 for UCP1, and P < 0.005 for UCP2 and UCP3). These results indicate that, even in a single bolus, leptin may regulate the mobilization and/or utilization of energy substrates such as fatty acids by affecting lipolytic activity in WAT and by increasing the expression of UCPs in BAT, WAT, and muscle.
Daisuke Tajima; Takayuki Masaki; Shuji Hidaka; Tetsuya Kakuma; Toshiie Sakata; Hironobu Yoshimatsu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  230     ISSN:  1535-3702     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-28     Completed Date:  2005-05-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  200-6     Citation Subset:  IM    
Department of Internal Medicine I, Faculty of Medicine, Oita University, Idaigaoka, Hasama, Oita, 879-5593 Japan.
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MeSH Terms
Adipose Tissue, Brown / metabolism
Base Sequence
Blood Glucose / metabolism
Carrier Proteins / genetics,  metabolism*
Fatty Acid-Binding Proteins
Fatty Acids / metabolism*
Gene Expression Regulation / drug effects
Glycerol / metabolism
Hypoglycemic Agents / pharmacology
Ion Channels
Leptin / pharmacology*
Lipolysis / drug effects*
Membrane Proteins / genetics,  metabolism*
Mitochondrial Proteins
Muscle, Skeletal / metabolism
Phosphotransferases / metabolism
RNA, Messenger / drug effects,  metabolism
Rats, Wistar
Sterol Esterase / metabolism
Reg. No./Substance:
0/Blood Glucose; 0/Carrier Proteins; 0/Fatty Acid-Binding Proteins; 0/Fatty Acids; 0/Hypoglycemic Agents; 0/Ion Channels; 0/Leptin; 0/Membrane Proteins; 0/Mitochondrial Proteins; 0/RNA, Messenger; 0/mitochondrial uncoupling protein; 56-81-5/Glycerol; EC 2.7.-/Phosphotransferases; EC Esterase

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