| Acute cell-mediated rejection in orthotopic pig-to-mouse corneal xenotransplantation. | |
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MedLine Citation:
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PMID: 19392722 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: To investigate the role of T cells and natural killer (NK) cells in mediating corneal xenograft rejection in a pig-to-mouse model. METHODS: Pig corneas were orthotopically transplanted into BALB/c, C57BL/6, nude, severe combined immunodeficiency (SCID), and NOD/SCID/gammac(null) (NOG) mice. Graft survival was clinically assessed by slit-lamp biomicroscopy and median survival times (MST) were calculated. The rejected grafts were histologically evaluated using antibodies against CD4, CD8, NK1.1, and F4/80. RESULTS: The pig corneal xenografts were acutely rejected by BALB/c and C57BL/6 mice (MST 9.0 days), while nude, SCID and NOG mice rejected pig corneas in a more delayed fashion (MST 16.0, 16.4, and 16.9 days, respectively). The majority of infiltrating cells found in rejected grafts in C57BL/6 mice were macrophages and CD4(+) T cells, while CD8(+) T cells and NK cells were rarely found. The grafts in nude mice had markedly decreased inflammatory infiltration with small numbers of macrophages and CD4(+) T cells. Infiltration was even more modest in grafts in SCID and NOG mice. CONCLUSIONS: T cells play an important role in acute rejection of pig corneal xenografts in mice, although acute rejection is not solely the result of T-cell-mediated immunity. NK cells are less likely to be involved in the rejection process. |
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Authors:
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Joo Youn Oh; Mee Kum Kim; Jung Hwa Ko; Hyun Ju Lee; Youngji Kim; Chan-Sik Park; Chung-Gyu Park; Sang Joon Kim; Won Ryang Wee; Jin Hak Lee |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Xenotransplantation Volume: 16 ISSN: 1399-3089 ISO Abbreviation: Xenotransplantation Publication Date: 2009 Mar-Apr |
Date Detail:
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Created Date: 2009-04-27 Completed Date: 2009-05-28 Revised Date: 2010-10-25 |
Medline Journal Info:
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Nlm Unique ID: 9438793 Medline TA: Xenotransplantation Country: Denmark |
Other Details:
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Languages: eng Pagination: 74-82 Citation Subset: IM |
Affiliation:
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Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, 28 Yeongeon-dong, Chongno-gu, Seoul, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cornea / pathology Corneal Transplantation / immunology* Graft Rejection / immunology* Graft Survival / immunology Humans Killer Cells, Natural / immunology* Mice Mice, Inbred BALB C / immunology Mice, Inbred C57BL / immunology Mice, Inbred NOD / immunology Mice, Nude / immunology Mice, SCID / immunology Sus scrofa / immunology T-Lymphocytes / immunology* Transplantation, Heterologous / immunology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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