Document Detail


Acute anorectic effect of single and combined drugs in mice using a non-deprivation protocol.
MedLine Citation:
PMID:  11594445     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RATIONALE: Studies of the effect of anorectic drugs such as fenfluramine in mice have indicated the desirability of using experimental protocols that do not involve deprivation. OBJECTIVE: We have developed a non-deprivation or "dessert" protocol for use in mice that are maintained in standard housing conditions, and examine the effects of a serotonergic agent dexfenfluramine (DFEN), a dopaminergic agent phentermine (PHEN), and a selective norepinephrine uptake inhibitor thionisoxetine (TNIX) alone and in combination. METHODS: Female C57BL/6J mice were adapted to 30 min daily presentation of a gelatinized form of sweetened milk using a holder that hooks over the side of the cage during tests; food spillage and contamination are minimal. Dose-inhibition curves were determined for DFEN, PHEN, and TNIX alone and for fixed ratio combinations of DFEN with either PHEN or TNIX. RESULTS: Each drug produced a near linear dose-inhibition curve with the 50% inhibitory doses (DI50) of 5.6, 3.2 and 12.2 mg/kg, respectively. By isobolographic analysis, the effects of the drug combinations were strictly additive. CONCLUSION: The procedure described is highly suitable for testing anorectic drugs in mice and is adaptable to a variety of housing conditions and diets. The DFEN+ PHEN combination was additive, which contrasts with its reported supra-additive effect in rats.
Authors:
N E Rowland; J Lo; K Robertson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Psychopharmacology     Volume:  157     ISSN:  0033-3158     ISO Abbreviation:  Psychopharmacology (Berl.)     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-10-11     Completed Date:  2002-02-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7608025     Medline TA:  Psychopharmacology (Berl)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  193-6     Citation Subset:  IM    
Affiliation:
Department of Psychology, University of Florida, Gainesville 32611-2250, USA. nrowland@ufl.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Appetite Depressants / administration & dosage*
Dexfenfluramine / administration & dosage
Drug Combinations
Eating / drug effects*,  physiology
Female
Fluoxetine / administration & dosage,  analogs & derivatives*
Food Deprivation* / physiology
Mice
Mice, Inbred C57BL
Phentermine / administration & dosage
Serotonin Agonists / administration & dosage
Chemical
Reg. No./Substance:
0/Appetite Depressants; 0/Drug Combinations; 0/Serotonin Agonists; 0/thionisoxetine; 122-09-8/Phentermine; 3239-44-9/Dexfenfluramine; 54910-89-3/Fluoxetine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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