Document Detail


Acute Plasmodium falciparum infection is associated with increased percentages of apoptotic cells.
MedLine Citation:
PMID:  7590929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The impact of acute malaria infection on the level of spontaneous apoptosis, i.e., the percentage of apoptotic cells detectable in lymphocytes cultured without any exogenous stimulus for 3 days in vitro, was evaluated. Quantitation of apoptosis was performed by staining of lymphocyte nuclei with propidium iodide and analysis of the fluorescence by cytometry. The mean apoptosis of 23 HIV-negative patients (15 Africans and 8 Europeans) determined during a confirmed Plasmodium falciparum attack was 27.2% (95% confidence interval (CI) = 23.5-30.7%) i.e., 2.2 times the mean level found in 49 controls (12.4%, CI = 11.1-13.6). These controls included age- and sex-matched Africans (n = 37) and Europeans (n = 12) differing only by their previous level of exposure to P. falciparum. Naive (European) as well as previously exposed (African) subjects showed dramatically elevated levels of spontaneous apoptosis during the malaria attack (mean = 22.5%, CI = 20.7-24.4 for Europeans; mean = 29.7%, CI = 24.6-34.7 for Africans). Such unusually raised levels were observed for at least 1.5 months and were probably detectable for longer periods as suggested by the fact that the mean level of spontaneous apoptosis in healthy Africans was basically higher (13.8%, CI = 12.5-15) than the one found in healthy Europeans (8.2%, CI = 6.3-10.1) (P = 0.0001). Selective immunomagnetic cell isolations carried out immediately before apoptosis quantitation showed that this process affected not only the alpha beta T cells (CD4+ T cells as well as CD8+ T cells) but also the gamma delta T cells and the B-lymphocyte subset.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
A T Baldé; J L Sarthou; C Roussilhon
Related Documents :
15325289 - Isolation and characterization of mammalian cells that are undergoing apoptosis by a bo...
10355539 - Mercuric chloride-induced apoptosis is dependent on protein synthesis.
20851479 - The defects in development and apoptosis of cardiomyocytes in mice lacking the transcri...
8827789 - Apoptotic cell death in the normal and cryptorchid human testis: the effect of human ch...
1436229 - Selective monocellular necrosis of hodgkin's/reed-sternberg cells and their immunoreact...
10946849 - Nifedipine induces apoptosis in cultured vascular smooth muscle cells from spontaneousl...
21547249 - Elucidating the complex interactions between stress and epileptogenic pathways.
15325289 - Isolation and characterization of mammalian cells that are undergoing apoptosis by a bo...
19429009 - Genetic modulation of apoptotic pathways fails to alter disease course in tripeptidyl-p...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Immunology letters     Volume:  46     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-12-07     Completed Date:  1995-12-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  59-62     Citation Subset:  IM; X    
Affiliation:
Unité d'Immunologie, Institut Pasteur, Dakar, Sénégal, West Africa.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acute Disease
Apoptosis / immunology*
Humans
Leukocytes, Mononuclear / physiology
Lymphocyte Count
Malaria, Falciparum / immunology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hybridoma cells producing antibodies against A-chain of mistletoe lectin I are resistant to this tox...
Next Document:  Phosphatidylinositol metabolism in lymphocytes of chronic alcoholic patients after anti-CD3 stimulat...