| Acute myocardial rescue with endogenous endothelial progenitor cell therapy. | |
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MedLine Citation:
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PMID: 20719564 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Post-myocardial infarction heart failure is a major health concern with limited therapy. Molecular revascularisation utilising granulocyte-macrophage colony stimulating factor (GMCSF) mediated endothelial progenitor cell (EPC) upregulation and stromal cell derived factor-1α (SDF) mediated myocardial EPC chemokinesis, may prevent myocardial loss and adverse remodelling. Vasculogenesis, viability, and haemodynamic improvements following therapy were investigated. PROCEDURES: Lewis rats (n=91) underwent LAD ligation and received either intramyocardial SDF and subcutaneous GMCSF or saline injections at the time of infarction. Molecular and haemodynamic assessments were performed at pre-determined time points following ligation. FINDINGS: SDF/GMCSF therapy upregulated EPC density as shown by flow cytometry (0.12±0.02% vs. 0.06±0.01% circulating lymphocytes, p=0.005), 48hours following infarction. A marked increase in perfusion was evident eight weeks after therapy, utilising confocal angiography (5.02±1.7×10(-2)μm(3)blood/μm(3)myocardial tissue vs. 2.03±0.710(-2)μm(3)blood/μm(3)myocardial tissue, p=0.00004). Planimetric analysis demonstrated preservation of wall thickness (0.98±0.09mm vs. 0.67±0.06mm, p=0.003) and ventricular diameter (7.81±0.99mm vs. 9.41±1.1mm, p=0.03). Improved haemodynamic function was evidenced by echocardiography and PV analysis (ejection fraction: 56.4±18.1% vs. 25.3±15.6%, p=0.001; pre-load adjusted maximal power: 6.6±2.6mW/μl(2) vs. 2.7±1.4mW/μl(2), p=0.01). CONCLUSION: Neovasculogenic therapy with GMCSF-mediated EPC upregulation and SDF-mediated EPC chemokinesis maybe an effective therapy for infarct modulation and preservation of myocardial function following acute myocardial infarction. |
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Authors:
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Pavan Atluri; Corinna M Panlilio; George P Liao; William Hiesinger; David Andrew Harris; Ryan C McCormick; Jeffrey E Cohen; Tao Jin; Wei Feng; Rebecca D Levit; Nianguo Dong; Y Joseph Woo |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-08-16 |
Journal Detail:
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Title: Heart, lung & circulation Volume: 19 ISSN: 1444-2892 ISO Abbreviation: Heart Lung Circ Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-18 Completed Date: 2011-03-18 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 100963739 Medline TA: Heart Lung Circ Country: Australia |
Other Details:
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Languages: eng Pagination: 644-54 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Australasian Society of Cardiac and Thoracic Surgeons and the Cardiac Society of Australia and New Zealand. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Division of Cardiovascular Surgery, Department of Surgery, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, United States. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Chemokine CXCL12 / pharmacology, therapeutic use* Echocardiography Endothelial Cells / drug effects* Flow Cytometry Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology, therapeutic use* Heart / drug effects* Heart Ventricles / anatomy & histology Hematopoietic Stem Cells / physiology Hemodynamics / drug effects* Male Models, Animal Myocardial Infarction / drug therapy* Myocardium / pathology Neovascularization, Physiologic / drug effects* Rats Rats, Inbred Lew Up-Regulation Ventricular Function, Left / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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1 F32 HL79769-01/HL/NHLBI NIH HHS; HL072812/HL/NHLBI NIH HHS; K08 HL072812-01A1/HL/NHLBI NIH HHS; R01 HL089315/HL/NHLBI NIH HHS; R01 HL089315-02/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CXCL12; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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