Document Detail

Acute exercise activates AMPK and eNOS in the mouse aorta.
MedLine Citation:
PMID:  21724864     Owner:  NLM     Status:  MEDLINE    
Exercise can prevent endothelial cell (EC) dysfunction and atherosclerosis even in the absence of improvements in plasma lipids. However, the mechanisms responsible for these effects are incompletely understood. In this study we examined in mice whether an acute bout of exercise activates enzymes that could prevent EC dysfunction, such as AMP-activated protein kinase (AMPK) and endothelial nitric oxide synthase (eNOS). We also examined whether exercise alters known regulators of these enzymes. C57BL/6 mice underwent a single bout of exhaustive treadmill exercise after which their aortas were analyzed for activation of AMPK, AMPK regulatory proteins, eNOS, and various enzymes that, like AMPK, activate eNOS. We found that such exercise acutely activates both AMPK and eNOS in the whole aorta and that the magnitude of these effects correlated with both the distance run and activation of the AMPK regulatory proteins silent information regulator-1 (SIRT1)-LKB1 and CaMKKβ. In contrast, Akt, PKA, PKG, and Src, other kinases known to activate eNOS, were unaffected. Immunohistochemical analysis revealed that AMPK and eNOS were both activated in the ECs of the aorta. This study provides the first evidence that an acute bout of exercise activates AMPK and eNOS in the endothelium of the aorta. The results also suggest that AMPK likely is the principal activator of eNOS in this setting and that its own activation may be mediated by both SIRT1-LKB1 and CaMKKβ.
José M Cacicedo; Marie-Soleil Gauthier; Nathan K Lebrasseur; Ravi Jasuja; Neil B Ruderman; Yasuo Ido
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-07-01
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  301     ISSN:  1522-1539     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-29     Completed Date:  2011-11-22     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1255-65     Citation Subset:  IM    
Diabetes and Metabolism Research Unit, Department of Medicine and Section of Endocrinology, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
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MeSH Terms
AMP-Activated Protein Kinases / metabolism*
Aorta, Thoracic / physiology*
Blotting, Western
Endothelial Cells / enzymology,  physiology
Endothelium, Vascular / enzymology,  physiology
Enzyme Activation / physiology
Mice, Inbred C57BL
Mice, Knockout
Nitric Oxide Synthase Type III / genetics,  metabolism*
Physical Conditioning, Animal / physiology*
Grant Support
P01-HL-68758-01-A6/HL/NHLBI NIH HHS; T32 DK-007201/DK/NIDDK NIH HHS
Reg. No./Substance:
EC Oxide Synthase Type III; EC Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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