Document Detail


Acute changes in circulating concentrations of progesterone and nitric oxide and partial pressure of oxygen during prostaglandin F2alpha-induced luteolysis in cattle.
MedLine Citation:
PMID:  19106483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To examine whether oxygen (O(2)) and nitric oxide (NO) are temporally associated with the acute changes in luteal function during luteolysis, we determined the real-time changes in the circulating concentrations of progesterone (P4) and nitrite/nitrate (the stable metabolites of NO) and the partial pressure of oxygen (pO(2)) during prostaglandin F(2alpha) (PGF(2alpha))-induced luteolysis in cattle. Catheters for frequent blood sample collection were inserted into the ovarian vein (OV), jugular vein (JV) and aorta abdominalis (AA) in 12 cows on Day 9 of the oestrous cycle (oestrus=Day 0). On Day 10, the cows were randomly divided into two groups and treated with a luteolytic dose of a PGF(2alpha) analogue or saline solution (control). Blood samples were collected at -2, -1, 0, 0.25, 0.5, 0.75, 1 and 2 h and then at 2-h intervals until 12 h after treatment (0 h). Injection of a PGF(2alpha) induced a significant decrease in the concentrations of P4 in OV plasma within 2 h. The decrease in P4 concentrations was preceded by an increase in the NO concentrations in the blood collected from OV, JV and AA. Basal pO(2) was significantly higher in OV blood than in JV blood (P<0.05). PGF(2alpha) injection increased pO(2) in OV blood between 0.5 and 2 h. These results demonstrate that PGF(2alpha) induced an acute increase in pO(2) and NO in the ovarian circulation and suggest that O(2) and NO are involved in the early events of CL regression, including inhibition of P4 secretion and output, in cattle.
Authors:
Tomas Javier Acosta; Mamadou Bousa Bah; Anna Korzekwa; Izabela Woclawek-Potocka; Wlodzimierz Markiewicz; Jerzy Jan Jaroszewski; Kiyoshi Okuda; Dariusz Jan Skarzynski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-24
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  55     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-05-13     Completed Date:  2009-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  149-55     Citation Subset:  IM    
Affiliation:
Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Gas Analysis / veterinary
Cattle / blood,  physiology*
Corpus Luteum / drug effects,  physiology*
Dinoprost / pharmacology*
Female
Luteolysis / drug effects,  physiology*
Nitric Oxide / blood*
Oxygen / blood*
Partial Pressure
Progesterone / blood*
Random Allocation
Chemical
Reg. No./Substance:
10102-43-9/Nitric Oxide; 551-11-1/Dinoprost; 57-83-0/Progesterone; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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