Document Detail


Acute Aerobic Exercise in Humans Increases Cytokine Expression in CD27- but not CD27+ CD8+ T-CELLS.
MedLine Citation:
PMID:  23017234     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Exercise alters the percentage of CD8(+)T-cells in the bloodstream expressing type 1 and type II cytokines. It is unknown if this reflects a change in cytokine expression within individual cells, or whether these observations result from the exercise-induced shift in the proportions of early/intermediate (CD27(+)) and late (CD27(-)) differentiated cells, which have vastly different cytokine profiles. 16 males cycled for 60 min at 95% maximal steady state. Mononuclear cells isolated from blood collected before, immediately after, and 1h after exercise were cultured overnight with and without phytohaemagglutinin stimulation. CD8(+)T-cells were assessed for differentiation markers and intracellular cytokine expression by flow cytometry. The numbers and percentage of CD27(-)CD8(+)T-cells increased immediately after exercise and fell below pre-exercise values 1h later. At 1h after exercise, an increased number and percentage of CD8(+)T-cells expressing IL-2, IFN-γ, TNF-α, IL-6, IL-4, and IL-10 was observed in both stimulated and unstimulated cells. The cytokine response to exercise was confined to CD27(-)CD8(+)T-cells, although cytokine expression among CD8(+)T-cells was highest when the proportion of CD27(-)CD8(+)T-cells was lowest. Moreover, the cytokine response to exercise could be predicted by the number of late cells in resting blood: cytokine expression was highest among those with low resting proportions of late cells. We conclude that exercise-induced changes in the percentage of CD8(+)T-cells expressing cytokines are not due to proportional shifts in early/intermediate and late differentiated T-cells. Exercise may prime late-differentiated blood CD8(+)T-cells to initiate effector functions in preparation for their extravasation into the tissues.
Authors:
Emily C Lavoy; Jos A Bosch; Thomas W Lowder; Richard J Simpson
Related Documents :
3053474 - Sympathoadrenergic regulation of metabolism and cardiocirculation during and following ...
16175014 - Variability in factor v:c assays in uk national external quality assessment scheme surv...
23294974 - The roles of aerobic exercise training and suppression il-6 gene expression by rna inte...
23538474 - Myocardial perfusion imaging in patients with a recent, normal exercise test.
6849264 - Spectrum of global left ventricular responses to supine exercise. limitation in the use...
23478474 - Performance analysis of professional, semi-professional and junior elite rugby league m...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-24
Journal Detail:
Title:  Brain, behavior, and immunity     Volume:  -     ISSN:  1090-2139     ISO Abbreviation:  Brain Behav. Immun.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8800478     Medline TA:  Brain Behav Immun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, Texas, USA 77204.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of murine anti-human Fab antibodies for use in an immunoassay for generic quantific...
Next Document:  African Plasmodium vivax: Distribution and origins.