| Acute Administration of Dopaminergic Drugs has Differential Effects on Locomotion in Larval Zebrafish. | |
| | |
MedLine Citation:
|
PMID: 23274813 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Altered dopaminergic signaling causes behavioral changes in mammals. In general, dopaminergic receptor agonists increase locomotor activity, while antagonists decrease locomotor activity. In order to determine if zebrafish (a model organism becoming popular in pharmacology and toxicology) respond similarly, the acute effects of drugs known to target dopaminergic receptors in mammals were assessed in zebrafish larvae. Larvae were maintained in 96-well microtiter plates (1 larva/well). Non-lethal concentrations (0.2-50 μM) of dopaminergic agonists (apomorphine, SKF-38393, and quinpirole) and antagonists (butaclamol, SCH-23390, and haloperidol) were administered at 6 days post-fertilization (dpf). An initial experiment identified the time of peak effect of each drug (20-260 minutes post-dosing, depending on the drug). Locomotor activity was then assessed for 70 minutes in alternating light and dark at the time of peak effect for each drug to delineate dose-dependent effects. All drugs altered larval locomotion in a dose-dependent manner. Both the D1- and D2-like selective agonists (SKF-38393 and quinpirole, respectively) increased activity, while the selective antagonists (SCH-23390 and haloperidol, respectively) decreased activity. Both selective antagonists also blunted the response of the larvae to changes in lighting conditions at higher doses. The nonselective drugs had biphasic effects on locomotor activity: apomorphine increased activity at the low dose and at high doses, while butaclamol increased activity at low to intermediate doses, and decreased activity at high doses. This study demonstrates that (1) larval zebrafish locomotion can be altered by dopamine receptor agonists and antagonists, (2) receptor agonists and antagonists generally have opposite effects, and (3) drugs that target dopaminergic receptors in mammals appear, in general, to elicit similar locomotor responses in zebrafish larvae. |
| | |
Authors:
|
T D Irons; P Kelly; D L Hunter; R C Macphail; S Padilla |
Related Documents
:
|
16581153 - Zotarolimus (abt-578) eluting stents. 17722543 - Nanoparticulate carriers for the treatment of coronary restenosis. 16884123 - Improving the safety of intravenous admixtures: lessons learned from a pentostam overdose. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-12-27 |
Journal Detail:
|
Title: Pharmacology, biochemistry, and behavior Volume: - ISSN: 1873-5177 ISO Abbreviation: Pharmacol. Biochem. Behav. Publication Date: 2012 Dec |
Date Detail:
|
Created Date: 2012-12-31 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0367050 Medline TA: Pharmacol Biochem Behav Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
|
Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; Integrated Systems Toxicology Division, National Health and Environmental Effects Laboratory, United States Environmental Protection Agency, Research Triangle Park, NC 27711. Electronic address: Terra.Irons@va.gov. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of the in vivo therapeutic properties of (-)-cubebin and (-)-hinokinin against Trypanosom...
Next Document: Population radiation dose from diagnostic nuclear medicine procedures in the tehran population in 19...