| Actual invasive potential of human hepatocellular carcinoma revealed by in situ gelatin zymography. | |
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MedLine Citation:
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PMID: 11751497 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The matrix-degrading proteinases are believed to play an important role in the invasion and metastasis of hepatocellular carcinoma (HCC), but no one has ever seen the in situ matrix-degrading activity in HCCs. PURPOSE: To demonstrate the cellular localization of actual gelatinolytic activity and to investigate the invasive potential of human HCC. EXPERIMENTAL DESIGN: HCC cases (30) were subjected to in situ gelatin zymography and SDS-gelatin gel zymogram. RESULTS: In situ gelatin zymography revealed a heterogeneous gelatinolytic activity in HCC cells, as well as stromal cells of noncancerous livers. The gelatinolytic intensity was stronger in 15 HCC nodules than in the corresponding noncancerous livers and was significantly associated with the cancer invasion to the capsule of the HCCs and to the portal veins. An intense gelatinolytic activity was detected in HCC cells in the front of tumor invasion. SDS-gelatin gel zymogram revealed gelatinases A and B that were mostly in latent forms. CONCLUSIONS: The present study demonstrates high gelatinolytic activity at the invasive front of HCCs at a cellular level and that HCC has an invasive potential with the gelatin (matrix)-degrading metalloproteinases. Furthermore, it suggests the importance of the activation mechanism of gelatinolytic enzymes in the invasion and metastasis of HCCs. |
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Authors:
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T Kaneyoshi; H Nakatsukasa; T Higashi; K Fujiwara; I Naito; K Nouso; K Kariyama; Y Kobayashi; M Uemura; S I Nakamura; Y Iwasaki; T Tsuji |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical cancer research : an official journal of the American Association for Cancer Research Volume: 7 ISSN: 1078-0432 ISO Abbreviation: Clin. Cancer Res. Publication Date: 2001 Dec |
Date Detail:
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Created Date: 2001-12-25 Completed Date: 2002-03-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9502500 Medline TA: Clin Cancer Res Country: United States |
Other Details:
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Languages: eng Pagination: 4027-32 Citation Subset: IM |
Affiliation:
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Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular
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enzymology,
pathology*,
surgery Cell Differentiation Electrophoresis, Polyacrylamide Gel Gelatin Gelatinases / analysis* Hepacivirus / isolation & purification Humans Isoenzymes / analysis Liver Neoplasms / enzymology, pathology*, surgery, virology Neoplasm Invasiveness / pathology* Neoplasm Staging Portal Vein / pathology Stromal Cells / pathology |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 9000-70-8/Gelatin; EC 3.4.24.-/Gelatinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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