| Actomyosin contractility and Discs large contribute to junctional conversion in guiding cell alignment within the Drosophila embryonic epithelium. | |
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MedLine Citation:
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PMID: 20332153 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Proper control of epithelial morphogenesis is vital to development and is often disrupted in disease. After germ band extension, the cells of the Drosophila ventral embryonic epidermis are packed in a two-dimensional polygonal array. Although epithelial cell rearrangements are being studied productively in several tissues, the ventral epidermis is of particular interest as the final cell arrangement is, uniquely, far from equilibrium. We show that over the course of several hours, a subset of cells within each parasegment adopts a rectilinear configuration and aligns into parallel columns. Live imaging shows that this is accomplished by the shrinkage of select cell interfaces, as three-cell junctions are converted to four-cell junctions. Additionally, we show that non-muscle Myosin II and the polarity proteins Discs large (Dlg) and Bazooka are enriched along cell interfaces in a complex but reproducible pattern that suggests their involvement in junctional conversion and cell alignment. Indeed, depletion of Myosin II or dlg disrupts these processes. These results show that tight spatial regulation of actomyosin contractility is required to produce this high-energy arrangement of cells. |
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Authors:
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Robert P Simone; Stephen DiNardo |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 137 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-24 Completed Date: 2010-04-23 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 1385-94 Citation Subset: IM |
Affiliation:
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University of Pennsylvania Medical School, Department of Cell and Developmental Biology, 421 Curie Boulevard, Philadelphia, PA 19104-6048, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actomyosin
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physiology* Animals Antibodies Cadherins / analysis Cell Shape Drosophila / embryology*, physiology Embryo, Nonmammalian / cytology, immunology, physiology Epidermis / cytology, physiology Epithelial Cells / cytology, physiology* Intercellular Junctions / physiology, ultrastructure Morphogenesis Myosin Type II / physiology |
| Grant Support | |
ID/Acronym/Agency:
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5T32HD007516/HD/NICHD NIH HHS; R01 GM045747-19/GM/NIGMS NIH HHS; R01 GM45747/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Cadherins; 9013-26-7/Actomyosin; EC 3.6.1.-/Myosin Type II |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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