Document Detail

Activity of thymidylate synthetase and its inhibition by 5-fluorouracil in highly enzyme-overproducing cells resistant to 10-propargyl-5,8-dideazafolate.
MedLine Citation:
PMID:  2528057     Owner:  NLM     Status:  MEDLINE    
Mouse FM3A mammary adenocarcinoma cells exposed to the specific thymidylate synthetase (TS) inhibitor 10-propargyl-5,8-dideazafolate (PDF) responded by overproducing TS up to 200-fold. In the absence of inhibitor, the elevation of TS levels decayed with a half-life of about 4 weeks. Southern blot analysis of restricted DNA from the PDF-resistant cells using a TS-specific probe showed that the TS gene was amplified to the same extent as enzyme levels. The PDF-resistant cells showed moderate cross-resistance to growth inhibition by 5-fluoro-2'-deoxyuridine, which increased with TS overproduction, but cross-resistance to 5-fluorouracil (FUra) was less (2- to 3-fold) and did not change with increased TS levels. TS activity, measured as release of tritium from [5-3H]2'-deoxyuridine, was no higher in the intact PDF-resistant cells than in wild-type cells. Inhibition of TS activity by FUra in the wild-type cells was accompanied by a proportional decrease in the amount of free TS, presumably due to formation of the tight binding complex of TS with 5-fluoro-2'-deoxyuridylate and 5,10'-methylenetetrahydrofolate. However, in the PDF-resistant cells, most the TS was still in the free form even though TS activity was substantially (85-90%) inhibited. Addition of folinic acid did not change either the sensitivity of the cells to FUra or the rates of tritium release in the cells having overproduced TS. These results are consistent with compartmentalization of TS, possibly in a multienzyme complex.
K D Danenberg; P V Danenberg
Related Documents :
307557 - Amphibian cells in culture. ii. isolation of drug-resistant variants and an asparagine-...
19528447 - Tissue transglutaminase promotes or suppresses tumors depending on cell context.
7694127 - Resistance of human larynx carcinoma cells to cisplatin, gamma-irradiation and methotre...
11276057 - Increased ribonucleotide reductase activity in hydroxyurea-resistant mosquito cells.
9421897 - Cytochemical studies of cell viability and gene expression in bacillus subtilis macrofi...
1820967 - Comparison of steroidogenic potencies of homologous and heterologous gonadotropins in r...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular pharmacology     Volume:  36     ISSN:  0026-895X     ISO Abbreviation:  Mol. Pharmacol.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-09-29     Completed Date:  1989-09-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0035623     Medline TA:  Mol Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  219-23     Citation Subset:  IM    
Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blotting, Southern
Drug Resistance
Floxuridine / pharmacology
Fluorouracil / pharmacology*
Folic Acid / analogs & derivatives*,  pharmacology
Gene Amplification
Quinazolines / pharmacology*
Thymidylate Synthase / antagonists & inhibitors*,  genetics
Tumor Cells, Cultured
Reg. No./Substance:
0/Quinazolines; 50-91-9/Floxuridine; 51-21-8/Fluorouracil; 59-30-3/Folic Acid; 76849-19-9/CB 3717; EC Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Restriction analysis of lambda EMBL3 background recombinants: occurrence of lambda phages carrying a...
Next Document:  Morphological, immunological and biochemical characterization of purified transverse tubule membrane...