| Activity and stability of an entrapped-cell system for the Delta(1)-dehydrogenation of steroids in organic media. | |
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MedLine Citation:
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PMID: 18601223 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Whole Arthrobacter simplex cells entrapped in kappa-carra-geenan or in two types of polyurethane foam, or adsorbed on silanized Celite, were tested for the Delta(1)-dehydrogenation of hydrocortisone and its derivatives in organic media. Catalytic activity and stability levels were evaluated for the immobilized cells in buffer with 2.5% (vol/vol) methanol, and in three buffer-saturated solvents (n-octan-1-ol, n-decan-1-ol, and chloroform). The addition of glutamate to the immobilization support stabilized the activity of the immobilized cells in the tested organic media. The system with polyurethane (HYPOL6100)-entrapped cells (with coimmobilized glutamate) in n-decan-1-ol provided the highest long-term activity levels. Several factors involved in the polyurethane-entrapment procedure were also studied. (c) 1992 John Wiley & Sons, Inc. |
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Authors:
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H M Pinheiro; J M Cabral |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 40 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 1992 Nov |
Date Detail:
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Created Date: 2008-07-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 1123-7 Citation Subset: - |
Affiliation:
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Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Av. Rovisco Pais, 1096 Lisbon, Portugal. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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