Document Detail

Activity of some mucolytics against bacterial adherence to mammalian cells.
MedLine Citation:
PMID:  18696263     Owner:  NLM     Status:  MEDLINE    
In this article, some mucolytic agents were tested for their activity to prevent bacterial adherence to mammalian cells. Preliminary screening for antiadherent activity showed that ambroxol, bromhexine, ammonium chloride, and ammonium acetate but neither guaiphenesin nor carbocysteine significantly reduced the adherence of the tested clinical isolates to cultured mammalian cells. The antiadherent effect of such agents was observed when mammalian cells were treated with these agents either before or after bacterial adherence, and this effect was exhibited in a dose-dependent manner. The minimum concentrations of ambroxol, bromhexine, ammonium chloride, and ammonium acetate required for mammalian cells treatment to prevent bacterial adherence were 2.5, 5, 50, and 20 ng/ml, respectively, whereas significantly higher mucolytic concentrations were required to eradicate bacteria that adhered to mammalian cells. Upon treatment of mammalian cells with mucolytics, the maximum reduction in adherence of the tested isolates attained by ambroxol, bromhexine, ammonium chloride, ammonium acetate were 99%, 98%, 75%, and 54% to that of control, respectively. Insignificant difference existed between the antiadherent activities of ambroxol and bromhexine, while both agents had significantly higher effect than ammonium chloride and ammonium acetate. Pretreatment of the immobilized mucin with ambroxol, bromhexine, ammonium chloride, or ammonium acetate reduced the adherence of Pseudomonas aeruginosa, Escherichia coli, and staphylococcal isolates to this receptor analogue. A strong correlation was observed for the antiadherent activity of the tested mucolytics in case of mammalian cells and immobilized mucin. Moreover, pretreatment of the immobilized receptor analogues chondroitin sulfate-B and heparin with the abovementioned agents significantly reduced the adherence of Staphylococcus aureus, P. aeruginosa, and E. coli isolates to such immobilized glycoproteins.
Mohamed M Hafez; Mohammad M Aboulwafa; Mahmoud A Yassien; Nadia A Hassouna
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Publication Detail:
Type:  Journal Article     Date:  2008-08-12
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  158     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-02     Completed Date:  2009-10-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  97-112     Citation Subset:  IM    
Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
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MeSH Terms
Bacteria / drug effects
Bacterial Adhesion / drug effects*
Cell Line
Dose-Response Relationship, Drug
Expectorants / pharmacology*
Reg. No./Substance:

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