| Activity of some mucolytics against bacterial adherence to mammalian cells. | |
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MedLine Citation:
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PMID: 18696263 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this article, some mucolytic agents were tested for their activity to prevent bacterial adherence to mammalian cells. Preliminary screening for antiadherent activity showed that ambroxol, bromhexine, ammonium chloride, and ammonium acetate but neither guaiphenesin nor carbocysteine significantly reduced the adherence of the tested clinical isolates to cultured mammalian cells. The antiadherent effect of such agents was observed when mammalian cells were treated with these agents either before or after bacterial adherence, and this effect was exhibited in a dose-dependent manner. The minimum concentrations of ambroxol, bromhexine, ammonium chloride, and ammonium acetate required for mammalian cells treatment to prevent bacterial adherence were 2.5, 5, 50, and 20 ng/ml, respectively, whereas significantly higher mucolytic concentrations were required to eradicate bacteria that adhered to mammalian cells. Upon treatment of mammalian cells with mucolytics, the maximum reduction in adherence of the tested isolates attained by ambroxol, bromhexine, ammonium chloride, ammonium acetate were 99%, 98%, 75%, and 54% to that of control, respectively. Insignificant difference existed between the antiadherent activities of ambroxol and bromhexine, while both agents had significantly higher effect than ammonium chloride and ammonium acetate. Pretreatment of the immobilized mucin with ambroxol, bromhexine, ammonium chloride, or ammonium acetate reduced the adherence of Pseudomonas aeruginosa, Escherichia coli, and staphylococcal isolates to this receptor analogue. A strong correlation was observed for the antiadherent activity of the tested mucolytics in case of mammalian cells and immobilized mucin. Moreover, pretreatment of the immobilized receptor analogues chondroitin sulfate-B and heparin with the abovementioned agents significantly reduced the adherence of Staphylococcus aureus, P. aeruginosa, and E. coli isolates to such immobilized glycoproteins. |
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Authors:
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Mohamed M Hafez; Mohammad M Aboulwafa; Mahmoud A Yassien; Nadia A Hassouna |
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Publication Detail:
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Type: Journal Article Date: 2008-08-12 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 158 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-07-02 Completed Date: 2009-10-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 97-112 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteria / drug effects Bacterial Adhesion / drug effects* Cell Line Dose-Response Relationship, Drug Expectorants / pharmacology* Humans |
| Chemical | |
Reg. No./Substance:
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0/Expectorants |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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