Document Detail


Activity of siRNAs with 2-thio-2'-O-methyluridine modification in mammalian cells.
MedLine Citation:
PMID:  20183560     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a search to identify chemical modifications to improve the properties of siRNA, we have investigated the effect of the 2 '-O-methyl-2-thiouridine modification on the biological activity of siRNA. Our results indicate that judicious placement of 2 '-O-methyl-2-thiouridine residues could lead to modified siRNA with activity in mammalian cells.
Authors:
Thazha P Prakash; Nishant Naik; Namir Sioufi; Balkrishen Bhat; Eric E Swayze
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleosides, nucleotides & nucleic acids     Volume:  28     ISSN:  1532-2335     ISO Abbreviation:  Nucleosides Nucleotides Nucleic Acids     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2010-02-25     Completed Date:  2010-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892832     Medline TA:  Nucleosides Nucleotides Nucleic Acids     Country:  England    
Other Details:
Languages:  eng     Pagination:  902-10     Citation Subset:  IM    
Affiliation:
Department of Medicinal Chemistry, Isis Pharmaceuticals Inc., Carlsbad, California 92008, USA. tprakashn@isisph.com
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Drug Design
Hela Cells
Humans
Oligonucleotides / chemical synthesis,  chemistry,  metabolism
RNA, Small Interfering / chemistry*,  genetics,  metabolism*
Thiouridine / analogs & derivatives*,  chemical synthesis,  chemistry,  metabolism
Chemical
Reg. No./Substance:
0/2'-O-methyl-2-thiouridine; 0/Oligonucleotides; 0/RNA, Small Interfering; 13957-31-8/Thiouridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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