| Activity of siRNAs with 2-thio-2'-O-methyluridine modification in mammalian cells. | |
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MedLine Citation:
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PMID: 20183560 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In a search to identify chemical modifications to improve the properties of siRNA, we have investigated the effect of the 2 '-O-methyl-2-thiouridine modification on the biological activity of siRNA. Our results indicate that judicious placement of 2 '-O-methyl-2-thiouridine residues could lead to modified siRNA with activity in mammalian cells. |
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Authors:
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Thazha P Prakash; Nishant Naik; Namir Sioufi; Balkrishen Bhat; Eric E Swayze |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nucleosides, nucleotides & nucleic acids Volume: 28 ISSN: 1532-2335 ISO Abbreviation: Nucleosides Nucleotides Nucleic Acids Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2010-02-25 Completed Date: 2010-06-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892832 Medline TA: Nucleosides Nucleotides Nucleic Acids Country: England |
Other Details:
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Languages: eng Pagination: 902-10 Citation Subset: IM |
Affiliation:
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Department of Medicinal Chemistry, Isis Pharmaceuticals Inc., Carlsbad, California 92008, USA. tprakashn@isisph.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Drug Design Hela Cells Humans Oligonucleotides / chemical synthesis, chemistry, metabolism RNA, Small Interfering / chemistry*, genetics, metabolism* Thiouridine / analogs & derivatives*, chemical synthesis, chemistry, metabolism |
| Chemical | |
Reg. No./Substance:
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0/2'-O-methyl-2-thiouridine; 0/Oligonucleotides; 0/RNA, Small Interfering; 13957-31-8/Thiouridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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