Document Detail

Activity of the plasma membrane H(+)-ATPase and optimal glycolytic flux are required for rapid adaptation and growth of Saccharomyces cerevisiae in the presence of the weak-acid preservative sorbic acid.
MedLine Citation:
PMID:  8795204     Owner:  NLM     Status:  MEDLINE    
The weak acid sorbic acid transiently inhibited the growth of Saccharomyces cerevisiae in media at low pH. During a lag period, the length of which depended on the severity of this weak-acid stress, yeast cells appeared to adapt to this stress, eventually recovering and growing normally. This adaptation to weak-acid stress was not due to metabolism and removal of the sorbic acid. A pma1-205 mutant, with about half the normal membrane H+-ATPase activity, was shown to be more sensitive to sorbic acid than its parent. Sorbic acid appeared to stimulate plasma membrane H+-ATPase activity in both PMA1 and pma1-205. Consistent with this, cellular ATP levels showed drastic reductions, the extent of which depended on the severity of weak-acid stress. The weak acid did not appear to affect the synthesis of ATP because CO2 production and O2 consumption were not affected significantly in PMA1 and pma1-205 cells. However, a glycolytic mutant, with about one-third the normal pyruvate kinase and phosphofructokinase activity and hence a reduced capacity to generate ATP, was more sensitive to sorbic acid than its isogenic parent. These data are consistent with the idea that adaptation by yeast cells to sorbic acid is dependent on (i) the restoration of internal pH via the export of protons by the membrane H+-ATPase in an energy-demanding process and (ii) the generation of sufficient ATP to drive this process and still allow growth.
C D Holyoak; M Stratford; Z McMullin; M B Cole; K Crimmins; A J Brown; P J Coote
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  62     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-10-17     Completed Date:  1996-10-17     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3158-64     Citation Subset:  IM    
Microbiology Department, Colworth Laboratory, Sharnbrook, Bedford, United Kingdom.
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MeSH Terms
Adaptation, Physiological
Adenosine Triphosphate / metabolism
Carbon Dioxide / metabolism
Food Preservatives / pharmacology*
Hydrogen-Ion Concentration
Oxygen Consumption
Proton-Translocating ATPases / metabolism*
Saccharomyces cerevisiae / drug effects*,  physiology
Sorbic Acid / pharmacology*
Reg. No./Substance:
0/Food Preservatives; 110-44-1/Sorbic Acid; 124-38-9/Carbon Dioxide; 56-65-5/Adenosine Triphosphate; EC ATPases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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