Document Detail


Activity of phosphatidylinositol-specific phospholipase C from Bacillus cereus with thiophosphate analogs of dimyristoylphosphatidylinositol.
MedLine Citation:
PMID:  9353901     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phosphatidylinositol-specific phospholipase C (PI-PLC) was studied with sonicated dispersions of a thiophosphate analog of phosphatidylinositol, 1, 2-dimyristoyloxypropane-3-thiophospho(1D-1-myo-inositol) (D-thio-DMPI). Kinetic parameters were derived from the rate as a function of bulk lipid concentration at constant saturating surface concentration of substrate (case I), and as a function of surface concentration of substrate at a constant saturating bulk concentration of lipid (case II). The substrate, D-thio-DMPI, was diluted with L-thio-DMPI or dimyristoyl phosphatidylmethanol (DMPM). In the presence of L-thio-DMPI, values for Vmax = 133 mumol min-1 mg-1, Ks' (the apparent dissociation constant for the enzyme-interface complex) = 0.097 mM, and Km* (the apparent interfacial Michaelis constant) = 0.22 mol fraction were obtained. DMPM caused enzyme inhibition in case I but no inhibition in case II. L-Thio-DMPI is an ideal neutral diluent with which to study the kinetics of PI-PLC.
Authors:
H S Hendrickson; A N Giles; S E Vos
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Chemistry and physics of lipids     Volume:  89     ISSN:  0009-3084     ISO Abbreviation:  Chem. Phys. Lipids     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1998-02-02     Completed Date:  1998-02-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0067206     Medline TA:  Chem Phys Lipids     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  45-53     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Washington, Seattle 98195, USA. hend@u.washington.edu
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MeSH Terms
Descriptor/Qualifier:
Bacillus cereus / enzymology*
Bacterial Proteins / metabolism*
Hydrolysis
Kinetics
Lipids / chemistry
Phosphatidylinositol Diacylglycerol-Lyase
Phosphatidylinositols / chemistry,  metabolism*
Phosphoinositide Phospholipase C
Phospholipids / chemistry
Sonication
Sulfhydryl Compounds / chemistry,  metabolism*
Type C Phospholipases / metabolism*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Lipids; 0/Phosphatidylinositols; 0/Phospholipids; 0/Sulfhydryl Compounds; 0/dimyristoyloxypropanethiophosphoinositol; EC 3.1.4.-/Type C Phospholipases; EC 3.1.4.11/Phosphoinositide Phospholipase C; EC 4.6.1.13/Phosphatidylinositol Diacylglycerol-Lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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