Document Detail


Activity of matrix metalloproteinase-2 and -9 and contents of their tissue inhibitors in uterine leiomyoma and corresponding myometrium.
MedLine Citation:
PMID:  17943549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND AIM: Matrix metalloproteinase-2 and -9 (MMP-2 and -9) are proteolytic enzymes degrading extracellular matrix proteins, mainly collagen type IV. Recent reports show that these proteases may be implicated in the growth of uterine leiomyoma. The aim of the present study was to evaluate the activity of MMP-2 and MMP-9, the contents of their tissue inhibitors (TIMP-1 and TIMP-2) and the immunolocalization of collagen type IV in uterine leiomyoma and corresponding myometrium. MATERIALS AND METHODS: Material for the study comprised specimens of uterine leiomyomas and corresponding myometrium derived from 20 hysterectomized women. The activity of MMP-2 and MMP-9 in tissue extracts was evaluated by semi-quantitative zymography. TIMPs were measured by enzyme-linked inmmunosorbent assay. Protein immunohistochemistry was applied for detection of collagen type IV. RESULTS: Activity and activation ratio of MMP-2 were significantly higher in leiomyomas than myometrium. The activity of MMP-9 was weak and did not differ between the investigated tissues. Contents of TIPM-1 and TIPM-2 were similar in both tissues. In both leiomyomas and myometrium, collagen type IV was localized in the extracellular matrix embedding bundles of smooth muscle cells, but was absent in areas of extracellular matrix accumulation within leiomyomas and in larger septa separating muscle fibers in normal myometrium. CONCLUSION: MMP-2 may be implicated in pathogenesis of leiomyoma.
Authors:
Michał Bogusiewicz; Marta Stryjecka-Zimmer; Krzysztof Postawski; Artur J Jakimiuk; Tomasz Rechberger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology     Volume:  23     ISSN:  0951-3590     ISO Abbreviation:  Gynecol. Endocrinol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-10-18     Completed Date:  2008-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807913     Medline TA:  Gynecol Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  541-6     Citation Subset:  IM    
Affiliation:
Second Department of Gynecology, Medical University of Lublin, Lubin, Poland. mbogusiewicz@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Collagen Type IV / metabolism
Female
Humans
Leiomyoma / enzymology,  metabolism*,  pathology
Matrix Metalloproteinase 2 / metabolism*,  physiology
Matrix Metalloproteinase 9 / metabolism*
Middle Aged
Myometrium / enzymology,  metabolism*,  pathology
Tissue Inhibitor of Metalloproteinase-1 / metabolism*
Tissue Inhibitor of Metalloproteinase-2 / metabolism*
Uterine Neoplasms / enzymology,  metabolism*,  pathology
Chemical
Reg. No./Substance:
0/Collagen Type IV; 0/Tissue Inhibitor of Metalloproteinase-1; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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