Document Detail


Activity of insulin growth factors and shrimp neurosecretory organ extracts on a lepidopteran cell line.
MedLine Citation:
PMID:  9055440     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ecdysteroids, or molting hormones, have been proven to be key differentiation regulators for epidermal cells in the postembryonic development of arthropods. Regulators of cell proliferation, however, remain largely unknown. To date, no diffusible insect peptidic growth factors have been characterized. Molecules structurally related to insulin have been discovered in insects, as in other eucaryotes. We developed in vitro tests for the preliminary characterization of potential growth factors in arthropods by adapting the procedures designed to detect such factors in vertebrates to an insect cell line (IAL-PID2) established from imaginal discs of the Indian meal moth. We verified the ability of these tests to measure the proliferation of IAL-PID2 cells. We tested mammalian insulin and insulin-like growth factors (IGF-I, IGF-II). Following an arrest of cell proliferation by serum deprivation, IGF-I and IGF-II caused partial resumption of the cell cycle, evidenced by DNA synthesis. In contrast, the addition of 20-hydroxyecdysone arrested the proliferation of the IAL-PID2 cells. The cell line was then used in a test for functional characterization of potential growth factors originating from the penaeid shrimp, Penaeus vannamei. Crude extracts of neurosecretory and nervous tissues, eyestalks, and ventral neural chain compensated for serum deprivation and stimulated completion of mitosis. Arch.
Authors:
P J Hatt; C Liebon; M Morinière; H Oberlander; P Porcheron
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of insect biochemistry and physiology     Volume:  34     ISSN:  0739-4462     ISO Abbreviation:  Arch. Insect Biochem. Physiol.     Publication Date:  1997  
Date Detail:
Created Date:  1997-04-02     Completed Date:  1997-04-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8501752     Medline TA:  Arch Insect Biochem Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  313-28     Citation Subset:  IM    
Affiliation:
ER 100 CNRS-URM 4 IFREMER, Départment de Biologie, Ecole Normale Supérieure, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Automation
Cell Cycle / drug effects
Cell Division / drug effects*
Cell Line
DNA / biosynthesis
Decapoda (Crustacea)*
Ecdysterone / pharmacology
Insects
Insulin-Like Growth Factor I / pharmacology*
Insulin-Like Growth Factor II / pharmacology*
Kinetics
Mammals
Neurosecretory Systems
Recombinant Proteins / pharmacology
Reproducibility of Results
Tissue Extracts / pharmacology*
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 0/Tissue Extracts; 5289-74-7/Ecdysterone; 67763-96-6/Insulin-Like Growth Factor I; 67763-97-7/Insulin-Like Growth Factor II; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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