| Activity-dependent neuroprotective protein: a novel gene essential for brain formation. | |
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MedLine Citation:
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PMID: 12888219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have recently cloned the novel homeobox-containing activity-dependent neuroprotective protein (ADNP). In the current study, mouse ADNP was shown to be expressed at the time of neural tube closure, detected at E7.5 and increased on E9.5. Expression was augmented in the brain (E12.5), sustained throughout embryogenesis and regulated by VIP. To assess the function of ADNP, knockout mice were established. Detailed analysis revealed cranial neural tube closure failure and death on E8.5-9.0 of the ADNP-knockout embryos. The expression of Oct4, a gene associated with germ-line maintenance was markedly augmented in the knockout embryos. In contrast, the expression of Pax6, a gene crucial for cerebral cortex formation, was abolished in the brain primordial tissue of the knockout embryos. Thus, Pax6 and Oct4 constitute a part of the mechanism of action of ADNP on brain formation, inhibiting germ-line division while activating morphogenesis. In conclusion, ADNP is identified here as a new key gene essential for organogenesis in the developing embryo and may be implicated as a clinical target associated with proper neurodevelopment. |
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Authors:
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Albert Pinhasov; Shmuel Mandel; Arkady Torchinsky; Eliezer Giladi; Zipora Pittel; Andrew M Goldsweig; Stephen J Servoss; Douglas E Brenneman; Illana Gozes |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Brain research. Developmental brain research Volume: 144 ISSN: 0165-3806 ISO Abbreviation: Brain Res. Dev. Brain Res. Publication Date: 2003 Aug |
Date Detail:
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Created Date: 2003-07-30 Completed Date: 2003-10-03 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8908639 Medline TA: Brain Res Dev Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 83-90 Citation Subset: IM |
Affiliation:
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Department of Clinical Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern / methods Blotting, Western / methods Brain / embryology*, metabolism Cloning, Molecular DNA-Binding Proteins / metabolism Embryo, Mammalian Embryonic and Fetal Development* Eye Proteins Gene Expression Regulation, Developmental* Gestational Age Homeodomain Proteins / metabolism In Situ Hybridization / methods Mice Mice, Inbred C57BL Mice, Knockout / embryology, genetics, metabolism Molecular Sequence Data Nerve Tissue Proteins / genetics, metabolism*, physiology Octamer Transcription Factor-3 Organ Culture Techniques / methods Paired Box Transcription Factors RNA, Messenger / biosynthesis, drug effects Repressor Proteins Reverse Transcriptase Polymerase Chain Reaction / methods Time Factors Transcription Factors* Vasoactive Intestinal Peptide / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Adnp protein, mouse; 0/DNA-Binding Proteins; 0/Eye Proteins; 0/Homeodomain Proteins; 0/Nerve Tissue Proteins; 0/Octamer Transcription Factor-3; 0/PAX6 protein; 0/Paired Box Transcription Factors; 0/Pou5f1 protein, mouse; 0/RNA, Messenger; 0/Repressor Proteins; 0/Transcription Factors; 37221-79-7/Vasoactive Intestinal Peptide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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