Document Detail


Activity of cecropin P1 and FA-LL-37 against urogenital microflora.
MedLine Citation:
PMID:  10955957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two mammalian antimicrobial peptides, FA-LL-37 and cecropin P1, were tested for activity against six uropathogens and five Lactobacillus strains by broth microdilution assay. Both peptides inhibited Escherichia coli at 25 microM (FA-LL-39), and 1.56 microM (cecropin P1), Pseudomonas aeruginosa (12.5 microM, and 25 microM), and Klebsiella pneumoniae, (50 microM, and 1.56 microM), but not Enterococcus faecalis and Staphylococcus epidermidis. FA-LL-37 acted bacteriocidally against E. coli and bacteriostatically against the other two Gram-negative organisms. Cecropin P1 was bacteriocidal to all susceptible bacteria. Lactobacilli were resistant to both peptides, with the exception of poultry isolate Lactobacillus fermentum B-54, which was susceptible to FA-LL-37 at 100 microM. The differential activities of these peptides toward Gram-negative uropathogens versus urogenital lactobacilli demonstrate their potential as a topical treatment for urinary tract infections. In addition, production of such peptides in vivo could be a natural mechanism to aid in the maintenance of the lactobacilli-dominated urogenital flora at the expense of pathogens.
Authors:
V Smeianov; K Scott; G Reid
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbes and infection / Institut Pasteur     Volume:  2     ISSN:  1286-4579     ISO Abbreviation:  Microbes Infect.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-11-15     Completed Date:  2001-02-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883508     Medline TA:  Microbes Infect     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  773-7     Citation Subset:  IM    
Affiliation:
Lawson Research Institute, University of Western Ontario, London, ON, Canada.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Anti-Bacterial Agents / pharmacology*
Antimicrobial Cationic Peptides*
Carrier Proteins / pharmacology*
Colony Count, Microbial
Enterobacteriaceae / drug effects*,  growth & development,  isolation & purification
Humans
Lactobacillus / drug effects*,  growth & development,  isolation & purification
Microbial Sensitivity Tests
Molecular Sequence Data
Peptides*
Pilot Projects
Urogenital System / microbiology*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Antimicrobial Cationic Peptides; 0/Carrier Proteins; 0/Peptides; 0/cathelicidin antimicrobial peptide; 0/cecropin P1-LI; 143108-26-3/CAP18 lipopolysaccharide-binding protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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