Document Detail

Activity cage as a method to analyze functional recovery after sciatic nerve injury in mice.
MedLine Citation:
PMID:  18097994     Owner:  NLM     Status:  MEDLINE    
The aim of this paper is to show the activity cage as a viable method for tracking functional nerve recovery. The activity cage measures spontaneous coordinate activity, meaning movement in either the horizontal or vertical plane, of experimental animals within a specified amount of time. This uses a minimum of researcher time conducting functional testing to determine functional recovery of the nerve. Using microsurgical forceps, a crush injury was inflicted unilaterally, on the left side, upon the 4-month-old C3H mice creating a very high degree of pressure for 6 s upon the exposed sciatic nerve. The locomotion function of the mice was evaluated using the activity cage preoperatively, 1, 7, 14, 21, and 28 days after the surgical procedure. We found that using the activity cage functional recovery occurred by 14 days after nerve crush injury. It was also shown that, coinciding with functional recovery, immunohistochemistry changes for GD1a and nNOS appeared at the level of L4, where the sciatic nerve joins the spinal column. GD1a and nNOS have both been linked to regenerative processes in mammalian nervous systems.
Roman Pavić; Ante Tvrdeić; Ozana Katarina Tot; Marija Heffer-Lauc
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Somatosensory & motor research     Volume:  24     ISSN:  0899-0220     ISO Abbreviation:  Somatosens Mot Res     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-21     Completed Date:  2008-03-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8904127     Medline TA:  Somatosens Mot Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  213-9     Citation Subset:  IM    
Department of Surgery, University Hospital Osijek, Osijek, Croatia.
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MeSH Terms
Behavioral Research / methods
Biological Markers / metabolism
Follow-Up Studies
Functional Laterality
Gangliosides / metabolism
Housing, Animal*
Lumbar Vertebrae
Mice, Inbred C3H
Motor Activity*
Nerve Crush*
Nerve Regeneration / physiology
Nitric Oxide Synthase Type I / metabolism
Recovery of Function*
Sciatic Nerve / injuries*,  metabolism,  physiopathology
Spinal Cord / metabolism
Reg. No./Substance:
0/Biological Markers; 0/Gangliosides; 12707-58-3/ganglioside, GD1a; EC Oxide Synthase Type I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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