Document Detail


Activity-based substrate profiling for Gcn5-related N-acetyltransferases: the use of chloroacetyl-coenzyme A to identify protein substrates.
MedLine Citation:
PMID:  17131985     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Gcn5-related N-acetyltransferases (GNAT) comprise one of the largest enzyme superfamilies, with over 10 000 known members represented in all kingdoms of life. ChloroacetylCoenzymeA was prepared and demonstrated to be a substrate for several GNAT members. ChloroacetylCoA (ClAcCoA) is used by the Hat1 histone acetyltransferase to correctly acetylate histone H4 in a mixture of histone proteins. Chloroacetylation can be assessed by the subsequent reaction of the chloroacetylated product with thiol-containing compounds, including those with fluorescent or affinity (His8) tags. The bacterial RimL N-acetyltransferase also uses ClAcCoA to chloroacetyl the alpha-amino group of its cognate substrate, the ribosomal L12 protein, and this reaction can be observed in crude extracts. ChloroacetylCoA is a reagent that can be used to identify the unknown substrate(s) for this large family of functionally uncharacterized enzymes.
Authors:
Michael Yu; Luiz Pedro Sorio de Carvalho; Guangxing Sun; John S Blanchard
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  128     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-29     Completed Date:  2007-02-01     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  15356-7     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetyl Coenzyme A / chemistry*
Acetylation
Bacterial Proteins / chemistry,  metabolism*
Histone Acetyltransferases / chemistry,  metabolism*
Protein Binding
Protein Conformation
Salmonella enterica / enzymology,  metabolism
Substrate Specificity
Grant Support
ID/Acronym/Agency:
AI60899/AI/NIAID NIH HHS; R01 AI060899-04/AI/NIAID NIH HHS; T32 GM07288/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 34201-15-5/chloroacetyl coenzyme A; 72-89-9/Acetyl Coenzyme A; EC 2.3.1.48/Histone Acetyltransferases
Comments/Corrections

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