| Activity-based substrate profiling for Gcn5-related N-acetyltransferases: the use of chloroacetyl-coenzyme A to identify protein substrates. | |
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MedLine Citation:
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PMID: 17131985 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Gcn5-related N-acetyltransferases (GNAT) comprise one of the largest enzyme superfamilies, with over 10 000 known members represented in all kingdoms of life. ChloroacetylCoenzymeA was prepared and demonstrated to be a substrate for several GNAT members. ChloroacetylCoA (ClAcCoA) is used by the Hat1 histone acetyltransferase to correctly acetylate histone H4 in a mixture of histone proteins. Chloroacetylation can be assessed by the subsequent reaction of the chloroacetylated product with thiol-containing compounds, including those with fluorescent or affinity (His8) tags. The bacterial RimL N-acetyltransferase also uses ClAcCoA to chloroacetyl the alpha-amino group of its cognate substrate, the ribosomal L12 protein, and this reaction can be observed in crude extracts. ChloroacetylCoA is a reagent that can be used to identify the unknown substrate(s) for this large family of functionally uncharacterized enzymes. |
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Authors:
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Michael Yu; Luiz Pedro Sorio de Carvalho; Guangxing Sun; John S Blanchard |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 128 ISSN: 0002-7863 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-11-29 Completed Date: 2007-02-01 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 15356-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetyl Coenzyme A
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chemistry* Acetylation Bacterial Proteins / chemistry, metabolism* Histone Acetyltransferases / chemistry, metabolism* Protein Binding Protein Conformation Salmonella enterica / enzymology, metabolism Substrate Specificity |
| Grant Support | |
ID/Acronym/Agency:
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AI60899/AI/NIAID NIH HHS; R01 AI060899-04/AI/NIAID NIH HHS; T32 GM07288/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 34201-15-5/chloroacetyl coenzyme A; 72-89-9/Acetyl Coenzyme A; EC 2.3.1.48/Histone Acetyltransferases |
| Comments/Corrections | |
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