Document Detail

Activity of Nidus Vespae extract and chemical fractions against Streptococcus mutans biofilms.
MedLine Citation:
PMID:  17916132     Owner:  NLM     Status:  MEDLINE    
AIMS: To evaluate the effect of Nidus Vespae extract and chemical fractions on the viability and architecture of Streptococcus mutans biofilms. METHODS AND RESULTS: The raw material was first extracted using 95% ethanol/water. Subsequent fractions were prepared from this extract using cyclohexane/ethyl acetate, petroleum ether/ethyl acetate and chloroform/methanol. The biomass dry weight and total protein of samples treated with Nidus Vespae extract and chemical fractions were significantly less than those treated with the vehicle control (P < 0.05). Biofilms treated with Nidus Vespae also resulted in lower percentage of polysaccharide composition. The pH decrease in the biofilm matrix was retarded by Nidus Vespae compared with the vehicle control. Architecture of biofilms treated with Nidus Vespae was different than in the vehicle control and 0.05% chlorhexidine. CONCLUSIONS: Chloroform/methanol fraction was the most effective treatment. SIGNIFICANCE AND IMPACT OF THE STUDY: The significant antibiofilm activity demonstrated by Nidus Vespae shows it to be a promising source of novel anticariogenic agents.
J Xiao; X D Zhou; J Feng; Y Q Hao; J Y Li
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-04
Journal Detail:
Title:  Letters in applied microbiology     Volume:  45     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-25     Completed Date:  2008-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  547-52     Citation Subset:  IM    
Key Laboratory for Oral Biomedical Engineering, Ministry of China.
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MeSH Terms
Anticarcinogenic Agents / isolation & purification,  pharmacology*
Biofilms / drug effects*
Chemical Fractionation
Chloroform / chemistry
Dental Plaque / microbiology*
Methanol / chemistry
Microscopy, Electron, Scanning
Streptococcus mutans / drug effects*,  physiology*
Wasps / chemistry*
Reg. No./Substance:
0/Anticarcinogenic Agents; 67-56-1/Methanol; 67-66-3/Chloroform

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