Document Detail

Activity of the RhoU/Wrch1 GTPase is critical for cranial neural crest cell migration.
MedLine Citation:
PMID:  21156169     Owner:  NLM     Status:  MEDLINE    
The neural crest (NC) is a stem cell-like population that arises at the border of neural and non-neural ectoderm. During development, NC undergoes an epithelio-mesenchymal transition (EMT), i.e. loss of epithelial junctions and acquisition of pro-migratory properties, invades the entire embryo and differentiates into a wide diversity of terminal tissues. We have studied the implication of Rho pathways in NC development and previously showed that RhoV is required for cranial neural crest (CNC) cell specification. We show here that the non-canonical Wnt response rhoU/wrch1 gene, closely related to rhoV, is also expressed in CNC cells but at later stages. Using both gain- and loss-of-function experiments, we demonstrate that the level of RhoU expression is critical for CNC cell migration and subsequent differentiation into craniofacial cartilages. In in vitro cultures, RhoU activates pathways that cooperate with PAK1 and Rac1 in epithelial adhesion, cell spreading and directional cell migration. These data support the conclusion that RhoU is an essential regulator of CNC cell migration.
Philippe Fort; Linda Guémar; Emmanuel Vignal; Nathalie Morin; Cécile Notarnicola; Pascal de Santa Barbara; Sandrine Faure
Related Documents :
2180649 - The proteins that control haemopoiesis and leukaemia.
6228579 - Human helper t cell factor(s). iii. characterization of b cell differentiation factor i...
3401619 - Biosynthesis of glycosaminoglycans in the human colonic tumor cell line caco-2: structu...
21176399 - Strategies for the development of cell lines for ex vivo gene therapy in the central ne...
11285139 - Coordinate regulation of rargamma2, tbp, and tafii135 by targeted proteolysis during re...
15572029 - Gdnf-induced leukemia inhibitory factor can mediate differentiation via the mek/erk pat...
8041899 - Radiation dose heterogeneity in receptor and antigen mediated boron neutron capture the...
21920629 - Changes in cysteine and o-acetyl-l-serine levels in the microalga chlorella sorokiniana...
22358759 - Suppressive effect of spinach extract on the formation of melanin in b16 mouse melanoma...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-11
Journal Detail:
Title:  Developmental biology     Volume:  350     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-31     Completed Date:  2011-03-28     Revised Date:  2011-04-06    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  451-63     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Universités Montpellier 2 et 1, CRBM, IFR122, Montpellier, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Movement*
Cell Polarity
Chick Embryo
Focal Adhesion Kinase 2 / physiology
Neural Crest / cytology*
Xenopus Proteins / physiology*
Xenopus laevis / embryology*
p21-Activated Kinases / physiology
rac GTP-Binding Proteins / physiology
rho GTP-Binding Proteins / physiology*
Reg. No./Substance:
0/Xenopus Proteins; EC Adhesion Kinase 2; EC Kinases; EC protein, Xenopus; EC GTP-Binding Proteins; EC GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Shox2 function couples neural, muscular and skeletal development in the proximal forelimb.
Next Document:  Further analyses of mechanisms underlying the antinociceptive effect of the triterpene 3?, 6?, 16?-t...