Document Detail

Activity of the RhoU/Wrch1 GTPase is critical for cranial neural crest cell migration.
MedLine Citation:
PMID:  21156169     Owner:  NLM     Status:  MEDLINE    
The neural crest (NC) is a stem cell-like population that arises at the border of neural and non-neural ectoderm. During development, NC undergoes an epithelio-mesenchymal transition (EMT), i.e. loss of epithelial junctions and acquisition of pro-migratory properties, invades the entire embryo and differentiates into a wide diversity of terminal tissues. We have studied the implication of Rho pathways in NC development and previously showed that RhoV is required for cranial neural crest (CNC) cell specification. We show here that the non-canonical Wnt response rhoU/wrch1 gene, closely related to rhoV, is also expressed in CNC cells but at later stages. Using both gain- and loss-of-function experiments, we demonstrate that the level of RhoU expression is critical for CNC cell migration and subsequent differentiation into craniofacial cartilages. In in vitro cultures, RhoU activates pathways that cooperate with PAK1 and Rac1 in epithelial adhesion, cell spreading and directional cell migration. These data support the conclusion that RhoU is an essential regulator of CNC cell migration.
Philippe Fort; Linda Guémar; Emmanuel Vignal; Nathalie Morin; Cécile Notarnicola; Pascal de Santa Barbara; Sandrine Faure
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-11
Journal Detail:
Title:  Developmental biology     Volume:  350     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-31     Completed Date:  2011-03-28     Revised Date:  2011-04-06    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  451-63     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Universités Montpellier 2 et 1, CRBM, IFR122, Montpellier, France.
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MeSH Terms
Cell Movement*
Cell Polarity
Chick Embryo
Focal Adhesion Kinase 2 / physiology
Neural Crest / cytology*
Xenopus Proteins / physiology*
Xenopus laevis / embryology*
p21-Activated Kinases / physiology
rac GTP-Binding Proteins / physiology
rho GTP-Binding Proteins / physiology*
Reg. No./Substance:
0/Xenopus Proteins; EC Adhesion Kinase 2; EC Kinases; EC protein, Xenopus; EC GTP-Binding Proteins; EC GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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