| Activin-A modulates gonadotropin-releasing hormone secretion from a gonadotropin-releasing hormone-secreting neuronal cell line. | |
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MedLine Citation:
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PMID: 1758579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The recent development of GnRH-secreting neuronal cell lines (GT1-1, GT1-3 and GT1-7 clones) has provided a model system for the study of the neural regulation of GnRH expression and secretion. We report here that activin-A stimulates GnRH secretion by GT1-7 cells in a dose-dependent manner, with an EC50 of approximately 2.5 ng/ml. The maximal response (50% stimulation) was achieved after 2 days of incubation with 20 ng/ml activin-A. Activin-A treatment increased total GnRH (secreted + cellular) in GT1-7 cells, possibly reflecting a stimulation of GnRH biosynthetic rates. The secretory effect of activin-A was also accompanied by a change in the cellular morphology to a more neuronal phenotype. The addition of TGF-beta (10 ng/ml), which is structurally related to activins, did not significantly increase secretion of GnRH by GT1-7 cells illustrating the specificity of the activin effect on this cell line. Although inhibin (20 ng/ml) alone did not directly affect the spontaneous secretion of GnRH, it was able to partially block the stimulatory effect of activin. The present study with the GT1-7 clonal cell line suggests that activin, and perhaps inhibin, might act at hypothalamic sites to regulate reproduction through the control of GnRH production and/or secretion. |
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Authors:
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C González-Manchón; L M Bilezikjian; A Z Corrigan; P L Mellon; W Vale |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neuroendocrinology Volume: 54 ISSN: 0028-3835 ISO Abbreviation: Neuroendocrinology Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1992-02-06 Completed Date: 1992-02-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0035665 Medline TA: Neuroendocrinology Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 373-7 Citation Subset: IM |
Affiliation:
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Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, Calif. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Activins Cell Line Clone Cells / secretion Gonadotropin-Releasing Hormone / secretion* Inhibins / physiology* Neurons / secretion* Radioimmunoassay Reproduction / physiology Transforming Growth Factor beta / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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HD-13527/HD/NICHD NIH HHS; HD-20377/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Transforming Growth Factor beta; 104625-48-1/Activins; 33515-09-2/Gonadotropin-Releasing Hormone; 57285-09-3/Inhibins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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