Document Detail


Activin-A modulates gonadotropin-releasing hormone secretion from a gonadotropin-releasing hormone-secreting neuronal cell line.
MedLine Citation:
PMID:  1758579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The recent development of GnRH-secreting neuronal cell lines (GT1-1, GT1-3 and GT1-7 clones) has provided a model system for the study of the neural regulation of GnRH expression and secretion. We report here that activin-A stimulates GnRH secretion by GT1-7 cells in a dose-dependent manner, with an EC50 of approximately 2.5 ng/ml. The maximal response (50% stimulation) was achieved after 2 days of incubation with 20 ng/ml activin-A. Activin-A treatment increased total GnRH (secreted + cellular) in GT1-7 cells, possibly reflecting a stimulation of GnRH biosynthetic rates. The secretory effect of activin-A was also accompanied by a change in the cellular morphology to a more neuronal phenotype. The addition of TGF-beta (10 ng/ml), which is structurally related to activins, did not significantly increase secretion of GnRH by GT1-7 cells illustrating the specificity of the activin effect on this cell line. Although inhibin (20 ng/ml) alone did not directly affect the spontaneous secretion of GnRH, it was able to partially block the stimulatory effect of activin. The present study with the GT1-7 clonal cell line suggests that activin, and perhaps inhibin, might act at hypothalamic sites to regulate reproduction through the control of GnRH production and/or secretion.
Authors:
C González-Manchón; L M Bilezikjian; A Z Corrigan; P L Mellon; W Vale
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroendocrinology     Volume:  54     ISSN:  0028-3835     ISO Abbreviation:  Neuroendocrinology     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1992-02-06     Completed Date:  1992-02-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0035665     Medline TA:  Neuroendocrinology     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  373-7     Citation Subset:  IM    
Affiliation:
Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, Calif.
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MeSH Terms
Descriptor/Qualifier:
Activins
Cell Line
Clone Cells / secretion
Gonadotropin-Releasing Hormone / secretion*
Inhibins / physiology*
Neurons / secretion*
Radioimmunoassay
Reproduction / physiology
Transforming Growth Factor beta / physiology*
Grant Support
ID/Acronym/Agency:
HD-13527/HD/NICHD NIH HHS; HD-20377/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Transforming Growth Factor beta; 104625-48-1/Activins; 33515-09-2/Gonadotropin-Releasing Hormone; 57285-09-3/Inhibins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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