Document Detail

Active transport of calcium across the isolated midgut of Hyalophora cecropia.
MedLine Citation:
PMID:  1003085     Owner:  NLM     Status:  MEDLINE    
1. The net flux of 45Ca from lumen to blood side across the isolated and short-circuited Cecropia midgut was 1-9 +/- 0-2 muequiv. cm-2h-1 in 8 mM Ca and the flux ratio was as high as 56 to 1. 2. The calcium influx was depressed by anoxia; 73% after 30 min. 3. The kinetics of Ca transport were anomalous; the apparent Km varied with Ca concentration from less than 0-2 to greater than 5-6 mM Ca and the apparent Vmax varied from less than 1-3 to greater than 3-3 muequiv. cm-2h-1. 4. The calcium influx showed a delay before the tracer steady state was attained, indicating the existence in the transport route of a calcium pool equivalent to 5-7 muequiv/g. wet weight of midgut tissue. 5 High calcium (16 mM) depressed the short-circuit current and potassium transport from blood to lumen side across the midgut. 6. Calcium depressed magnesium transport, from lumen to blood side across the midgut, and magnesium depressed the calcium transport. 7. Ca transport by the midgut does not regulate the Ca level in the haemolymph in vivo; it merely aids the diffusion of calcium down its electrochemical gradient. However, Ca transport may assist the uptake of the nutrients from the midgut contents.
J L Wood; W R Harvey
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  65     ISSN:  0022-0949     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  1976 Oct 
Date Detail:
Created Date:  1977-02-24     Completed Date:  1977-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  347-60     Citation Subset:  IM    
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MeSH Terms
Biological Transport, Active
Bombyx / metabolism*
Calcium / metabolism*
Intestines / metabolism*
Magnesium / metabolism
Oxygen / pharmacology
Potassium / metabolism
Reg. No./Substance:
7439-95-4/Magnesium; 7440-09-7/Potassium; 7440-70-2/Calcium; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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