Document Detail

Active tissue factor shed from human arterial smooth muscle cells adheres to artificial surfaces.
MedLine Citation:
PMID:  11263809     Owner:  NLM     Status:  MEDLINE    
Through a series of in vitro assays, this study outlines a flow-mediated process by which active tissue factor (TF), the prime initiator of coagulation, may be transferred from the plasma membrane of vascular smooth muscle cells (VSMCs) to that of artificial surfaces such as those typically associated with intravascular implants. Studies with quiescent and activated rat VSMCs demonstrated that pathologically high shear stresses (tau(w) = 250 dyn cm(-2)) resulted in the loss of TF activity from the cell surface. Subsequent experiments with human VSMCs showed that VSMCs continuously release active TF into their extracellular medium, presumably in the form of lipid vesicles or microparticles, and that fluid shear stress (tauw = 50 dyncm(-2)) or chemical agonists (A23187) can significantly accelerate this release. Experiments with a wide array of polymeric and metallic materials showed that the TF shed from VSMCs was able to adhere to these surfaces and promote the activation of coagulation factor X (FX) at the material surface. Extracellular TF bound strongly to both uncoated and human plasma coated surfaces under a wide range of hemodynamic shear stresses (0-20 dyncm(-2)). When an extracellular, VSMC-derived TF mixture was perfused over Ti 6-4 surfaces, the adhesion of TF was found to be time-dependent, gradually accumulating on the material surface over time. Thus an important criterion in the design or success of intravascular devices may be related to their ability to interact with TF, shed from cell surfaces. This is especially important as TF may lead to thrombotic complications, the products of which may also increase cellular proliferation.
J J Hathcock; C L Hall; V T Turitto
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biomaterials science. Polymer edition     Volume:  11     ISSN:  0920-5063     ISO Abbreviation:  J Biomater Sci Polym Ed     Publication Date:  2000  
Date Detail:
Created Date:  2001-03-23     Completed Date:  2001-09-27     Revised Date:  2008-02-20    
Medline Journal Info:
Nlm Unique ID:  9007393     Medline TA:  J Biomater Sci Polym Ed     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1211-25     Citation Subset:  IM    
Department of Biomedical Engineering, The University of Memphis, TN 38152, USA.
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MeSH Terms
Blood Flow Velocity
Coated Materials, Biocompatible / metabolism*
Factor X / metabolism
Muscle, Smooth, Vascular / metabolism*
Protein Binding
Stress, Mechanical
Thromboplastin / metabolism*
Grant Support
Reg. No./Substance:
0/Coated Materials, Biocompatible; 9001-29-0/Factor X; 9035-58-9/Thromboplastin

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