| Active and passive mechanisms of helicases. | |
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MedLine Citation:
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PMID: 20423906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this work, we discuss the active or passive character of helicases. In the past years, several studies have used the theoretical framework proposed by Betterton and Julicher [Betterton, M.D. and Julicher, F. (2005) Opening of nucleic-acid double strands by helicases: active versus passive opening. Phys. Rev. E, 71, 11904-11911.] to analyse the unwinding data and assess the mechanism of the helicase under study (active versus passive). However, this procedure has given rise to apparently contradictory interpretations: helicases exhibiting similar behaviour have been classified as both active and passive enzymes [Johnson, D.S., Bai, L. Smith, B.Y., Patel, S.S. and Wang, M.D. (2007) Single-molecule studies reveal dynamics of DNA unwinding by the ring-shaped T7 helicase. Cell, 129, 1299-1309; Lionnet, T., Spiering, M.M., Benkovic, S.J., Bensimon, D. and Croquette, V. (2007) Real-time observation of bacteriophage T4 gp41 helicase reveals an unwinding mechanism Proc. Natl Acid. Sci., 104, 19790-19795]. In this work, we show that when the helicase under study has not been previously well characterized (namely, if its step size and rate of slippage are unknown) a multi-parameter fit to the afore-mentioned model can indeed lead to contradictory interpretations. We thus propose to differentiate between active and passive helicases on the basis of the comparison between their observed translocation velocity on single-stranded nucleic acid and their unwinding rate of double-stranded nucleic acid (with various GC content and under different tensions). A threshold separating active from passive behaviour is proposed following an analysis of the reported activities of different helicases. We study and contrast the mechanism of two helicases that exemplify these two behaviours: active for the RecQ helicase and passive for the gp41 helicase. |
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Authors:
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Maria Manosas; Xu Guang Xi; David Bensimon; Vincent Croquette |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-27 |
Journal Detail:
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Title: Nucleic acids research Volume: 38 ISSN: 1362-4962 ISO Abbreviation: Nucleic Acids Res. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-13 Completed Date: 2010-10-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: England |
Other Details:
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Languages: eng Pagination: 5518-26 Citation Subset: IM |
Affiliation:
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Laboratoire de Physique Statistique, Ecole Normale Superieure, UPMC Univ Paris 06, Universit Paris Diderot, CNRS, 24 rue Lhomond, 75005 Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biocatalysis DNA / chemistry, metabolism DNA Helicases / metabolism* Models, Biological RecQ Helicases / metabolism* Viral Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Viral Proteins; 0/gene 41 protein, Enterobacteria phage T4; 9007-49-2/DNA; EC 3.6.1.-/DNA Helicases; EC 3.6.1.-/RecQ Helicases; EC 3.6.1.-/RecQ protein, E coli |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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