Document Detail


Active, motor-driven mechanics in a DNA gel.
MedLine Citation:
PMID:  23045635     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells are capable of a variety of dramatic stimuli-responsive mechanical behaviors. These capabilities are enabled by the pervading cytoskeletal network, an active gel composed of structural filaments (e.g., actin) that are acted upon by motor proteins (e.g., myosin). Here, we describe the synthesis and characterization of an active gel using noncytoskeletal components. We use methods of base-pair-templated DNA self assembly to create a hybrid DNA gel containing stiff tubes and flexible linkers. We then activate the gel by adding the motor FtsK50C, a construct derived from the bacterial protein FtsK that, in vitro, has a strong and processive DNA contraction activity. The motors stiffen the gel and create stochastic contractile events that affect the positions of attached beads. We quantify the fluctuations of the beads and show that they are comparable both to measurements of cytoskeletal systems and to theoretical predictions for active gels. Thus, we present a DNA-based active gel whose behavior highlights the universal aspects of nonequilibrium, motor-driven networks.
Authors:
Olivier J N Bertrand; Deborah Kuchnir Fygenson; Omar A Saleh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-10-08
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-24     Completed Date:  2013-01-07     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17342-7     Citation Subset:  IM    
Affiliation:
Département de Physique, École Normale Supérieure, 75005 Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Biomechanics
DNA / chemistry*
Gels
Molecular Motor Proteins / chemistry
Chemical
Reg. No./Substance:
0/Gels; 0/Molecular Motor Proteins; 9007-49-2/DNA
Comments/Corrections

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