Document Detail

Active lytic infection of human primary tonsillar B cells by KSHV and its noncytolytic control by activated CD4+ T cells.
MedLine Citation:
PMID:  21339648     Owner:  NLM     Status:  MEDLINE    
Kaposi sarcoma-associated herpesvirus (KSHV) is a B-lymphotropic virus whose primary site of replication is the oropharynx. KSHV can infect both T and B cells from primary tonsillar explant cultures. However, T cells do not support lytic replication, while B cells spontaneously produce substantial amounts of infectious virus. Here, we provide evidence for a mechanism by which activated T cells may promote or stabilize latency of KSHV infection in B cells. When mixed cultures of B cells and activated T cells were exposed to KSHV, little spontaneous virus production was observed. Removing T cells from the mix or treating the mixed culture with immune suppressants enhanced virus production. Adding back activated T cells to purified infected B cells efficiently suppressed KSHV production, primarily due to CD4(+) T cells. This suppressive activity required T cell activation and direct cell-cell contact, but not prior exposure to KSHV antigen. Suppression was not MHC restricted and did not result in killing of the target cell. We therefore propose that oropharyngeal T cells activated by a variety of stimuli can recognize ligands on infected target B cells, leading to signaling events that prevent spontaneous lytic activation and promote latent infection in this compartment.
Jinjong Myoung; Don Ganem
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-21
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  121     ISSN:  1558-8238     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-02     Completed Date:  2011-04-27     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1130-40     Citation Subset:  AIM; IM    
Howard Hughes Medical Institute, Department of Microbiology, UCSF, San Francisco, California 94143, USA.
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MeSH Terms
B-Lymphocytes / virology*
CD4-Positive T-Lymphocytes / virology*
Child, Preschool
Gene Expression Regulation, Viral*
Green Fluorescent Proteins / metabolism
Herpesvirus 8, Human / metabolism*
Lymphocyte Activation
Palatine Tonsil / metabolism,  virology*
Signal Transduction
Virus Latency / genetics
Grant Support
P01DE019085/DE/NIDCR NIH HHS; //Howard Hughes Medical Institute
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins
Comment In:
J Clin Invest. 2011 Mar;121(3):838-41   [PMID:  21339638 ]

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