Document Detail


Active lipoprotein precursors in the Gram-positive eubacterium Lactococcus lactis.
MedLine Citation:
PMID:  12584195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipid-modified proteins play important roles at the interface between eubacterial cells and their environment. The importance of lipoprotein processing by signal peptidase II (SPase II) is underscored by the fact that this enzyme is essential for viability of the Gram-negative eubacterium Escherichia coli. In contrast, SPase II is not essential for growth and viability of the Gram-positive eubacterium Bacillus subtilis. This could be due to alternative amino-terminal lipoprotein processing, which was shown previously to occur in SPase II mutants of B. subtilis. Alternatively, uncleaved lipoprotein precursors might be functional. To explore further the importance of lipoprotein processing in Gram-positive eubacteria, an SPase II mutant strain of Lactococcus lactis was constructed. Although some of the 39 (predicted) lactococcal lipoproteins, such as PrtM and OppA, are essential for growth in milk, the growth of SPase II mutant L. lactis cells in this medium was not affected. Furthermore, the activity of the strictly PrtM-dependent extracellular protease PrtP, which is required for casein degradation, was not impaired in the absence of SPase II. Importantly, no alternative processing of pre-PrtM and pre-OppA was observed in cells lacking SPase II. Taken together, these findings show for the first time that authentic lipoprotein precursors retain biological activity.
Authors:
Roelke Venema; Harold Tjalsma; Jan Maarten van Dijl; Anne de Jong; Kees Leenhouts; Girbe Buist; Gerard Venema
Related Documents :
221035 - Structural organization of free and esterified cholesterol in human high density lipopr...
10357505 - Lipid reactivity to stress: i. comparison of chronic and acute stress responses in midd...
22833655 - Maternal low-protein diet induces gender-dependent changes in epigenetic regulation of ...
2043215 - Hyperlipoproteinemias: part i. lipoprotein classification and abnormalities.
21912105 - Relationship between the effect of dietary fat on swimming endurance and energy metabol...
23528875 - Enhanced intestinal epithelial barrier health status on european sea bass (dicentrarchu...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-02-12
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  278     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-21     Completed Date:  2003-07-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14739-46     Citation Subset:  IM    
Affiliation:
Department of Genetics, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, Kerklaan 30, 9751 NN Haren, The Netherlands.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U63724
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aspartic Acid Endopeptidases / genetics,  physiology
Bacterial Proteins / biosynthesis,  physiology
Base Sequence
Carrier Proteins / biosynthesis,  physiology
Cell Division
Lactococcus lactis / chemistry*,  growth & development
Lipoproteins / biosynthesis*,  physiology
Membrane Proteins / biosynthesis,  physiology
Molecular Sequence Data
Protein Precursors*
Protein Processing, Post-Translational*
Protein Sorting Signals
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Carrier Proteins; 0/Lipoproteins; 0/Membrane Proteins; 0/Protein Precursors; 0/Protein Sorting Signals; 0/PrtM protein, Lactococcus lactis; 0/oligopeptide-binding protein, bacteria; EC 3.4.23.-/Aspartic Acid Endopeptidases; EC 3.4.23.36/signal peptidase II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The ALD6 gene product is indispensable for providing NADPH in yeast cells lacking glucose-6-phosphat...
Next Document:  Recycling of apoprotein E is associated with cholesterol efflux and high density lipoprotein interna...