| Active lipids of Ganoderma lucidum spores-induced apoptosis in human leukemia THP-1 cells via MAPK and PI3K pathways. | |
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MedLine Citation:
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PMID: 22172327 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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ETHNOPHARMACOLOGICAL RELEVANCE: Ganoderma lucidum (Lingzhi) is traditionally drug, which has been traditionally effective used in the treatment of chronic hepatopathy, hypertension, hyperglycemia and cancer. AIM OF THE STUDY: Ganoderma lucidum has been used extensively as a folk remedy for its anti-tumor activity. The present study aims to elucidate the signaling mechanisms involved in active lipids of Ganoderma lucidum spores-induced THP-1 cells apoptosis by investigating the role of MAPK, PI3K and caspase-3, -8 and -9. MATERIALS AND METHODS: THP-1 and HL-60 apoptosis was quantified by flow cytometry using FITC-conjugated annexin V and PI; MAPK and Akt were measured by Western blot, and caspase-3, -8 and -9 activities were also detected by spectrophotometric assay. RESULTS: Our results showed that active lipids of Ganoderma lucidum spores decreased phosphorylation-ERK1/2 (P-ERK1/2), P-Akt and increased P-JNK1/2, but did not affect expressions of P-p38 MAPK in THP-1 cells. Moreover, treatment of THP-1 cells with active lipids of Ganoderma lucidum spores resulted in activation of caspase-3, -8 and -9. Furthermore, LY294002 (Akt inhibitor) or PD98059 (ERK1/2 inhibitor) significantly enhanced active lipids of Ganoderma lucidum spores-induced apoptosis in THP-1 cells, whereas caspase inhibitors or SP600125 (JNK inhibitor), decreased apoptosis in THP-1 cells. CONCLUSION: Taken together, our study for the first time suggests that active lipids of Ganoderma lucidum spores is able to enhance apoptosis in THP-1 cells, at least in part, through inhibition of ERK1/2, Akt and activation of JNK1/2 signaling pathways. Moreover, it also triggers caspase-3, -8 and -9 activation mediated apoptotic induction. |
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Authors:
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Jia-He Wang; Yi-Jun Zhou; Meng Zhang; Liang Kan; Ping He |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-6 |
Journal Detail:
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Title: Journal of ethnopharmacology Volume: - ISSN: 1872-7573 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7903310 Medline TA: J Ethnopharmacol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ireland Ltd. |
Affiliation:
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Department of Geriatrics, Shengjing Hospital of China Medical University Shenyang, Shenyang 110004, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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