Document Detail


Active cell movements coupled to positional induction are involved in lineage segregation in the mouse blastocyst.
MedLine Citation:
PMID:  19422818     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the mouse blastocyst, some cells of the inner cell mass (ICM) develop into primitive endoderm (PE) at the surface, while deeper cells form the epiblast. It remained unclear whether the position of cells determines their fate, such that gene expression is adjusted to cell position, or if cells are pre-specified at random positions and then sort. We have tracked and characterised dynamics of all ICM cells from the early to late blastocyst stage. Time-lapse microscopy in H2B-EGFP embryos shows that a large proportion of ICM cells change position between the surface and deeper compartments. Most of this cell movement depends on actin and is associated with cell protrusions. We also find that while most cells are precursors for only one lineage, some give rise to both, indicating that lineage segregation is not complete in the early ICM. Finally, changing the expression levels of the PE marker Gata6 reveals that it is required in surface cells but not sufficient for the re-positioning of deeper cells. We provide evidence that Wnt9A, known to be expressed in the surface ICM, facilitates re-positioning of Gata6-expressing cells. Combining these experimental results with computer modelling suggests that PE formation involves both cell sorting movements and position-dependent induction.
Authors:
Sigolène M Meilhac; Richard J Adams; Samantha A Morris; Anne Danckaert; Jean-François Le Garrec; Magdalena Zernicka-Goetz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-05
Journal Detail:
Title:  Developmental biology     Volume:  331     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-29     Completed Date:  2009-09-30     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  210-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Differentiation / metabolism
Blastocyst Inner Cell Mass / cytology*,  physiology
Body Patterning / physiology
Cell Differentiation / physiology
Cell Lineage / physiology*
Cell Movement / physiology*
Endoderm / cytology,  embryology,  physiology
GATA6 Transcription Factor / metabolism
Mice
Models, Biological
Wnt Proteins / metabolism
Grant Support
ID/Acronym/Agency:
064421//Wellcome Trust; G0300723//Medical Research Council; G0400709//Medical Research Council; G0800784//Medical Research Council; //Biotechnology and Biological Sciences Research Council; //Wellcome Trust
Chemical
Reg. No./Substance:
0/Antigens, Differentiation; 0/GATA6 Transcription Factor; 0/Gata6 protein, mouse; 0/Wnt Proteins; 0/Wnt9a protein, mouse
Comments/Corrections

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