Document Detail


Active RNA polymerases: mobile or immobile molecular machines?
MedLine Citation:
PMID:  20644712     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is widely assumed that active RNA polymerases track along their templates to produce a transcript. We test this using chromosome conformation capture and human genes switched on rapidly and synchronously by tumour necrosis factor alpha (TNFalpha); one is 221 kbp SAMD4A, which a polymerase takes more than 1 h to transcribe. Ten minutes after stimulation, the SAMD4A promoter comes together with other TNFalpha-responsive promoters. Subsequently, these contacts are lost as new downstream ones appear; contacts are invariably between sequences being transcribed. Super-resolution microscopy confirms that nascent transcripts (detected by RNA fluorescence in situ hybridization) co-localize at relevant times. Results are consistent with an alternative view of transcription: polymerases fixed in factories reel in their respective templates, so different parts of the templates transiently lie together.
Authors:
Argyris Papantonis; Joshua D Larkin; Youichiro Wada; Yoshihiro Ohta; Sigeo Ihara; Tatsuhiko Kodama; Peter R Cook
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-13
Journal Detail:
Title:  PLoS biology     Volume:  8     ISSN:  1545-7885     ISO Abbreviation:  PLoS Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-20     Completed Date:  2010-10-26     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101183755     Medline TA:  PLoS Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1000419     Citation Subset:  IM    
Affiliation:
Sir William Dunn School of Pathology, Medical Sciences Division, University of Oxford, Oxford, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Base Pairing / genetics
Cells, Cultured
Chromosomes, Human, Pair 14 / genetics
DNA-Directed RNA Polymerases / metabolism*
Enzyme Activation / drug effects
Humans
In Situ Hybridization, Fluorescence
Introns / genetics
Protein Binding / drug effects
RNA Transport / drug effects
Repressor Proteins / metabolism
Time Factors
Transcription, Genetic / drug effects
Transcriptional Activation / drug effects,  genetics
Tumor Necrosis Factor-alpha / pharmacology
Grant Support
ID/Acronym/Agency:
BVROJG0//Wellcome Trust
Chemical
Reg. No./Substance:
0/Repressor Proteins; 0/SAMD4A protein, human; 0/Tumor Necrosis Factor-alpha; EC 2.7.7.6/DNA-Directed RNA Polymerases
Comments/Corrections

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