Document Detail


Activators of the glutamate-dependent acid resistance system alleviate deleterious effects of YidC depletion in Escherichia coli.
MedLine Citation:
PMID:  21216990     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The function of the essential inner membrane protein (IMP) YidC in Escherichia coli has been studied for a limited number of model IMPs and primarily using targeted approaches. These studies suggested that YidC acts at the level of insertion, folding, and quality control of IMPs, both in the context of the Sec translocon and as a separate entity. To further our understanding of YidC's role in IMP biogenesis, we screened a random overexpression library for factors that rescued the growth of cells upon YidC depletion. We found that the overexpression of the GadX and GadY regulators of the glutamate-dependent acid resistance system complemented the growth defect of YidC-depleted cells. Evidence is presented that GadXY overexpression counteracts the deleterious effects of YidC depletion on at least two fronts. First, GadXY prepares the cells for the decrease in respiratory capacity upon the depletion of YidC. Most likely, GadXY-regulated processes reduce the drop in proton-motive force that impairs the fitness of YidC-depleted cells. Second, in GadXY-overproducing cells increased levels of the general chaperone GroEL cofractionate with the inner membranes, which may help to keep newly synthesized inner membrane proteins in an insertion-competent state when YidC levels are limiting.
Authors:
Zhong Yu; Martijn Bekker; Angela Tramonti; Gregory M Cook; Peter van Ulsen; Dirk-Jan Scheffers; Joost Teixeira de Mattos; Daniela De Biase; Joen Luirink
Publication Detail:
Type:  Journal Article     Date:  2011-01-07
Journal Detail:
Title:  Journal of bacteriology     Volume:  193     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-25     Completed Date:  2011-04-18     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1308-16     Citation Subset:  IM    
Affiliation:
Department of Molecular Microbiology, Institute of Molecular Cell Biology, VU University Amsterdam, Amsterdam 1081 HV, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
AraC Transcription Factor / biosynthesis*
Chaperonin 60 / metabolism
Escherichia coli / growth & development,  metabolism,  physiology*
Escherichia coli Proteins / biosynthesis*,  metabolism*
Gene Expression*
Genetic Complementation Test
Membrane Transport Proteins / metabolism*
Microbial Viability
RNA, Small Untranslated / biosynthesis*
Transcription Factors / biosynthesis*
Chemical
Reg. No./Substance:
0/AraC Transcription Factor; 0/Chaperonin 60; 0/Escherichia coli Proteins; 0/GadX protein, E coli; 0/Membrane Transport Proteins; 0/RNA, Small Untranslated; 0/Transcription Factors; 0/YIDC protein, E coli
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