| Activation of the ubiquitin proteolytic pathway in human septic heart and diaphragm. | |
| | |
MedLine Citation:
|
PMID: 19375358 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: Mechanisms of sepsis-induced myocardial and diaphragmatic alteration are multiple and remain largely unknown, particularly in humans. In the present study, we compared the inducible nitric oxide synthase (NOS-2) expression, the peroxynitrite production and the expression and activation of the ubiquitin proteolytic pathway in the wall of the 4 cardiac chambers, in the diaphragm, and in the rectus abdominis. PATIENTS: Seven patients who died from septic shock associated with a myocardial depression and 5 nonseptic (control) patients. MEASUREMENTS AND RESULTS: We evaluated protein expression by Western blot. Nitrotyrosin and ubiquitin residues were localized by immunofluorescence. NOS-2, nitrated proteins, free ubiquitin, and ubiquitinated proteins are overexpressed in the wall of the four cardiac cavities, in the diaphragm and in the rectus abdominis of septic patients at a similar level. Ubiquitinated proteins with a molecular mass of 50, 35, 30, and 25 kD were consistently detected in heart, diaphragm, and rectus abdominis of septic shock patients but lacking in nonseptic patients. In situ immunolabelling of ubiquitin showed a colocalisation with nitrotyrosine residues at the sarcomeric level of cardiac myocytes in septic patients. CONCLUSIONS: This study showed the first evidence for the activation of the proteolytic ubiquitin-proteasome pathway in human heart and diaphragm in septic shock. |
| | |
Authors:
|
Christophe Rabuel; Jane-Lise Samuel; Brice Lortat-Jacob; Françoise Marotte; Sophie Lanone; Christine Keyser; Arrigo Lessana; Didier Payen; Alexandre Mebazaa |
Related Documents
:
|
11947978 - Equivalence of the standard monophasic waveform shocks delivered by automated external ... 18496368 - Actual incidence of global left ventricular hypokinesia in adult septic shock. 16459008 - The characteristics of postcountershock pulseless electrical activity may indicate the ... 11071798 - Lack of progress in cardiogenic shock: lessons from the gusto trials. 6814818 - Nitroglycerin combined with methoxamine in experimental coronary occlusions. 20837908 - Effect of thrombin fragment (tp508) on myocardial ischemia reperfusion injury in a mode... |
Publication Detail:
|
Type: Journal Article Date: 2009-04-16 |
Journal Detail:
|
Title: Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology Volume: 19 ISSN: 1879-1336 ISO Abbreviation: Cardiovasc. Pathol. Publication Date: 2010 May-Jun |
Date Detail:
|
Created Date: 2010-05-10 Completed Date: 2010-08-13 Revised Date: 2011-10-27 |
Medline Journal Info:
|
Nlm Unique ID: 9212060 Medline TA: Cardiovasc Pathol Country: United States |
Other Details:
|
Languages: eng Pagination: 158-64 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Anesthesiology and Critical Care Medicine, Lariboisière Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Aged, 80 and over Blotting, Western Diaphragm / metabolism*, pathology Female Fluorescent Antibody Technique Humans Male Middle Aged Myocardium / metabolism*, pathology Nitric Oxide Synthase Type II / metabolism Peroxynitrous Acid / metabolism Rectus Abdominis / metabolism, pathology Shock, Septic / metabolism*, pathology Signal Transduction Ubiquitin / metabolism* Ubiquitinated Proteins / metabolism* Ubiquitination |
| Chemical | |
Reg. No./Substance:
|
0/Ubiquitin; 0/Ubiquitinated Proteins; 14691-52-2/Peroxynitrous Acid; EC 1.14.13.39/NOS2 protein, human; EC 1.14.13.39/Nitric Oxide Synthase Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nonseptal right atrial aneurysm: case report and review of literature.
Next Document: Preservation of mitochondrial integrity and energy metabolism during experimental status epilepticus...