Document Detail

Activation of uPAR Is Required for Cigarette Smoke Extract-Induced Epithelial-Mesenchymal Transition in Lung Epithelial Cells.
MedLine Citation:
PMID:  25198659     Owner:  NLM     Status:  In-Data-Review    
Cigarette smoke is a major risk factor for lung cancer, which may contribute to lung cancer invasion and metastasis. However, the mechanism remains unclear. Epithelial-mesenchymal transition (EMT) is a critical phenotypic alteration of cells that triggers invasion and metastasis. The urokinase-type plasminogen activator receptor (uPAR) is originally thought to assist the directional invasion of migrating cells, and increasing evidences show that overexpression of uPAR in cancer cells promotes EMT. Therefore, we intend to study the role of uPAR in cigarette smoke extract (CSE)-induced EMT in lung epithelial cells. In this study, we showed that lung epithelial cells cultured after CSE treatment demonstrated changes consistent with EMT. E-cadherin was decreased, while vimentin, N-cadherin, and α-SMA expression was increased in both A549 and BEAS-2B cells. Cells acquired a mesenchymal-like morphology and increased cell motility and invasion. In addition, CSE-induced EMT was accompanied by increased expression of uPAR and activation of AKT downstream of uPAR. CSE-induced EMT and activation of AKT were blocked by uPAR gene silencing. Antagonizing PI3K also inhibits development of CSE-induced EMT. We conclude that CSE can induce EMT, and the activity of uPAR-dependent signal pathway in EMT is recapitulated in lung epithelial cells in vitro.
Qin Wang; Hongchao Wang; Yi Zhang; Yuke Zhang; Wei Xiao
Related Documents :
3415639 - Isolation of mouse isometallothioneins. a comparison of isometallothioneins in growing ...
10993269 - Hürthle cell tumours of the thyroid. a review with emphasis on mitochondrial abnormalit...
1526289 - Chemoimmunotherapy of cancer: cyclophosphamide-resistant tumour cells are equally sensi...
2748939 - Cell proliferation and differentiation in squamous cancer.
19840029 - Effect of syndecan-1 overexpression on mesenchymal tumour cell proliferation with focus...
15047749 - Expression of connective tissue growth factor (ccn2) in desmoplastic small round cell t...
20335519 - Spontaneous in vitro transformation of primary human osteoblast-like cells.
18541369 - Phenol degradation by aureobasidium pullulans fe13 isolated from industrial effluents.
9572289 - Regulation of nicotinic receptor subtypes following chronic nicotinic agonist exposure ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oncology research     Volume:  21     ISSN:  1555-3906     ISO Abbreviation:  Oncol. Res.     Publication Date:  2013  
Date Detail:
Created Date:  2014-09-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208097     Medline TA:  Oncol Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  295-305     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Regulation of MET Kinase Inhibitor Resistance by Copy Number of MET in Gastric Carcinoma Cells.
Next Document:  Epigenetically Modified Pancreatic Carcinoma PANC-1 Cells Can Act as Cancer Vaccine to Enhance Antit...