Document Detail


Activation of uPAR Is Required for Cigarette Smoke Extract-Induced Epithelial-Mesenchymal Transition in Lung Epithelial Cells.
MedLine Citation:
PMID:  25198659     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Cigarette smoke is a major risk factor for lung cancer, which may contribute to lung cancer invasion and metastasis. However, the mechanism remains unclear. Epithelial-mesenchymal transition (EMT) is a critical phenotypic alteration of cells that triggers invasion and metastasis. The urokinase-type plasminogen activator receptor (uPAR) is originally thought to assist the directional invasion of migrating cells, and increasing evidences show that overexpression of uPAR in cancer cells promotes EMT. Therefore, we intend to study the role of uPAR in cigarette smoke extract (CSE)-induced EMT in lung epithelial cells. In this study, we showed that lung epithelial cells cultured after CSE treatment demonstrated changes consistent with EMT. E-cadherin was decreased, while vimentin, N-cadherin, and α-SMA expression was increased in both A549 and BEAS-2B cells. Cells acquired a mesenchymal-like morphology and increased cell motility and invasion. In addition, CSE-induced EMT was accompanied by increased expression of uPAR and activation of AKT downstream of uPAR. CSE-induced EMT and activation of AKT were blocked by uPAR gene silencing. Antagonizing PI3K also inhibits development of CSE-induced EMT. We conclude that CSE can induce EMT, and the activity of uPAR-dependent signal pathway in EMT is recapitulated in lung epithelial cells in vitro.
Authors:
Qin Wang; Hongchao Wang; Yi Zhang; Yuke Zhang; Wei Xiao
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oncology research     Volume:  21     ISSN:  1555-3906     ISO Abbreviation:  Oncol. Res.     Publication Date:  2013  
Date Detail:
Created Date:  2014-09-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208097     Medline TA:  Oncol Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  295-305     Citation Subset:  IM    
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