Document Detail


Activation of serotonergic neurons during salicylate-induced tinnitus.
MedLine Citation:
PMID:  21192277     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
HYPOTHESIS: Serotonergic neurons are activated during salicylate-induced tinnitus and modulate the cochlea during tinnitus.
BACKGROUND: During salicylate-induced tinnitus in the gerbil, neurons in the dorsal raphe nucleus were activated. Because approximately half of the neurons in this region are serotonergic, this indicates that serotonin (5-HT) might play a role in the mechanisms of central tinnitus. The goal of this study was to determine if serotonergic neurons are activated during salicylate-induced tinnitus. Furthermore, to determine if the same neurons might modulate the cochlea during tinnitus, neuroanatomic tract-tracing with 5-HT immunohistochemistry was used to determine if serotonergic neurons project to the gerbil cochlea.
METHODS: A randomized, prospective study was performed. Six gerbils were injected with salicylate (saline for controls). Four hours later, the gerbils were euthanized and perfused, and their brains were collected for immunohistochemical labeling of 5-HT and c-fos. For the tract-tracing, FluoroGold was injected into the cochleae of 3 gerbils. The gerbils were euthanized and perfused 4 to 11 days later, and the brains immunohistochemically were processed for 5-HT.
RESULTS: More serotonergic neurons expressed c-fos in the salicylate-injected animals compared with the controls. The increase was significant for 3 of the 8 major serotonergic cell groups including B7, B9, and the caudal linear nucleus. Despite robust labeling of olivocochlear and vestibular efferents with FluoroGold, 5-HT-labeled neurons containing FluoroGold were lacking.
CONCLUSION: Salicylate-induced tinnitus activates serotonergic neurons in rostral cell groups. Activation of these neurons is not likely to influence cochlear function directly but is likely to influence a number of auditory and nonauditory regions known to be involved with tinnitus.
Authors:
Kimberly K Caperton; Ann M Thompson
Related Documents :
15749347 - Major sex differences in non-genomic estrogen actions on intracellular signaling in mou...
770157 - Effect of hypophysectomy on immunocytochemically demonstrated gonadotropin-releasing ho...
12239467 - The spatial relationship of gamma-aminobutyric acid (gaba) neurons and gonadotropin-rel...
21262457 - The multiple faces of rim.
17444757 - Reinforcement learning through modulation of spike-timing-dependent synaptic plasticity.
3808467 - Relationship of parasagittal bands of acetylcholinesterase activity to the climbing fib...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology     Volume:  32     ISSN:  1537-4505     ISO Abbreviation:  Otol. Neurotol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100961504     Medline TA:  Otol Neurotol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  301-7     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA. Kimberly-Caperton@ouhsc.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Audiometric outcomes for acoustic neuroma patients after single versus multiple fraction stereotacti...
Next Document:  Role of estrogen receptor-dependent upregulation of P38 MAPK/heme oxygenase 1 in resveratrol-mediate...