Document Detail

Activation of serotonergic neurons during salicylate-induced tinnitus.
MedLine Citation:
PMID:  21192277     Owner:  NLM     Status:  In-Process    
HYPOTHESIS: Serotonergic neurons are activated during salicylate-induced tinnitus and modulate the cochlea during tinnitus.
BACKGROUND: During salicylate-induced tinnitus in the gerbil, neurons in the dorsal raphe nucleus were activated. Because approximately half of the neurons in this region are serotonergic, this indicates that serotonin (5-HT) might play a role in the mechanisms of central tinnitus. The goal of this study was to determine if serotonergic neurons are activated during salicylate-induced tinnitus. Furthermore, to determine if the same neurons might modulate the cochlea during tinnitus, neuroanatomic tract-tracing with 5-HT immunohistochemistry was used to determine if serotonergic neurons project to the gerbil cochlea.
METHODS: A randomized, prospective study was performed. Six gerbils were injected with salicylate (saline for controls). Four hours later, the gerbils were euthanized and perfused, and their brains were collected for immunohistochemical labeling of 5-HT and c-fos. For the tract-tracing, FluoroGold was injected into the cochleae of 3 gerbils. The gerbils were euthanized and perfused 4 to 11 days later, and the brains immunohistochemically were processed for 5-HT.
RESULTS: More serotonergic neurons expressed c-fos in the salicylate-injected animals compared with the controls. The increase was significant for 3 of the 8 major serotonergic cell groups including B7, B9, and the caudal linear nucleus. Despite robust labeling of olivocochlear and vestibular efferents with FluoroGold, 5-HT-labeled neurons containing FluoroGold were lacking.
CONCLUSION: Salicylate-induced tinnitus activates serotonergic neurons in rostral cell groups. Activation of these neurons is not likely to influence cochlear function directly but is likely to influence a number of auditory and nonauditory regions known to be involved with tinnitus.
Kimberly K Caperton; Ann M Thompson
Related Documents :
25431077 - In vivo assessment of neuronal cell death in drosophila drosophila.
15749347 - Major sex differences in non-genomic estrogen actions on intracellular signaling in mou...
7982507 - On the distribution of catecholamines in promesostoma balticum (turbellaria, neorhabdoc...
15363947 - Neuroendocrinology of gastric h+ and duodenal hco3- secretion: the role of brain-gut axis.
9159217 - The pattern and mechanism of mitochondrial transport in axons.
3123007 - Relative size of the anterior thalamic nuclei differentiates anthropoids by social system.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology     Volume:  32     ISSN:  1537-4505     ISO Abbreviation:  Otol. Neurotol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100961504     Medline TA:  Otol Neurotol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  301-7     Citation Subset:  IM    
Department of Otorhinolaryngology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Audiometric outcomes for acoustic neuroma patients after single versus multiple fraction stereotacti...
Next Document:  Role of estrogen receptor-dependent upregulation of P38 MAPK/heme oxygenase 1 in resveratrol-mediate...