| Activation of resistance arteries with endothelin-1: from vasoconstriction to functional adaptation and remodeling. | |
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MedLine Citation:
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PMID: 15017111 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Remodeling of resistance arteries is a key feature in hypertension. We studied the transition of vasoconstriction to remodeling in isolated rat skeletal muscle arterioles. Arterioles activated with 10 nM endothelin-1 showed functional adaptation when kept at low distension in a wire myograph setup, where contractile properties shifted towards a smaller lumen diameter after 1 day. Pressurized arteries kept in organoid culture showed physical inward remodeling after 3-day activation with 10 nM endothelin-1, characterized by a reduction in relaxed diameter without a change in the wall cross-sectional area (eutrophic remodeling). The relaxed lumen diameter (at 60 mm Hg) decreased from 169 +/- 5 (day 0) to 155 +/- 4 microm (day 3). An antibody directed to the beta(3)-integrin subunit (but not one directed to the beta(1)-integrin subunit) enhanced remodeling, from a reduction in relaxed diameter at 60 mm Hg of 15 +/- 2.4 to 22 +/- 1.8 microm (both on day 3). Collagen gel contraction experiments showed that the antibody directed to the beta(3)-integrin subunit enhanced the compaction of collagen by smooth muscle cells, from 83 +/- 1.5 to 68 +/- 1.5% of the initial gel diameter. In conclusion, these data show that inward eutrophic remodeling is a response to sustained contraction, which may involve collagen reorganization through beta(3)-integrins. |
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Authors:
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Erik N T P Bakker; Carsten L Buus; Ed VanBavel; Michael J Mulvany |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-03-12 |
Journal Detail:
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Title: Journal of vascular research Volume: 41 ISSN: 1018-1172 ISO Abbreviation: J. Vasc. Res. Publication Date: 2004 Mar-Apr |
Date Detail:
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Created Date: 2004-04-15 Completed Date: 2004-05-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9206092 Medline TA: J Vasc Res Country: Switzerland |
Other Details:
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Languages: eng Pagination: 174-82 Citation Subset: IM |
Copyright Information:
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Copyright 2004 S. Karger AG, Basel |
Affiliation:
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Department of Pharmacology, University of Aarhus, Aarhus, Denmark. n.t.bakker@amc.uva.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological
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drug effects,
physiology* Animals Antibodies, Monoclonal / pharmacology Antigens, CD29 / immunology, metabolism Aorta / cytology, metabolism* Endothelin-1 / pharmacology* Extracellular Matrix / metabolism Integrin beta3 / immunology, metabolism MAP Kinase Signaling System / drug effects, physiology Male Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases / metabolism Muscle, Smooth, Vascular / cytology, enzymology* Organ Culture Techniques Rats Rats, Wistar Vascular Resistance / drug effects, physiology* Vasoconstriction / drug effects, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, CD29; 0/Endothelin-1; 0/Integrin beta3; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 2.7.11.24/Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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