| Activation and proliferation of follicular dendritic cell-like cells by activated T lymphocytes. | |
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MedLine Citation:
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PMID: 7522246 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Follicular dendritic cell (FDC)-like cell lines (HK cells) from human tonsils were established to investigate the functional role of FDC in the germinal centers of lymphoid follicles. Although HK cells exhibited CD21, CD23, DRC-1, CD40, VCAM-1, ICAM-1, and HJ2 that were expressed on human tonsilar FDC at early stages of establishment, they lost DRC-1, CD21, and CD23 within 3 days of culture. Morphologic and functional characterization studies suggest that HK cells are quite distinct from fibroblasts. The growth requirement of HK cells is different from the previously reported FDC-like cell lines. Functionally, these cells bound B cells but not T cells, and supported B cell proliferation. The culture supernatants of HK cells had costimulatory activity on the proliferation of anti-mu- or anti-CD40-activated B cells, and the activity dramatically increased after 12-O-tetradecanoylphorbol 13-acetate stimulation. Interestingly, anti-CD3 Ab activated T cell bound to HK cells, inducing the phenotypic changes and the growth of HK cells. The T-HK cell interactions not only involve the well known adhesion ligand/receptor pathways (VLA-4/VCAM-1 and LFA-1/ICAM-1), but also involve CD40-CD40 ligand as shown by inhibitory effect of soluble CD40 (CD40.Fc). The cellular interactions between T and HK cells and cytokine production suggest that activated T cells not only stimulate resting B cells directly, but also support B cell maturation indirectly by stimulating the development of FDC. Hence HK cells may be useful in identifying the surface molecules and cytokines of FDC involved in the GC formation. |
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Authors:
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H S Kim; X Zhang; Y S Choi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 153 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1994 Oct |
Date Detail:
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Created Date: 1994-10-14 Completed Date: 1994-10-14 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2951-61 Citation Subset: AIM; IM |
Affiliation:
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Cellular Immunology Laboratory, Alton Ochsner Medical Foundation, New Orleans, LA 70121. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / physiology Antigens, CD40 Antigens, Differentiation, B-Lymphocyte / physiology Antigens, Surface / analysis B-Lymphocytes / cytology* Cell Adhesion Molecules / physiology Cell Communication Cell Division Cell Line Cytokines / pharmacology Dendritic Cells / cytology, immunology* Immunophenotyping Lymph Nodes / cytology Lymphocyte Activation Mice Palatine Tonsil / cytology*, immunology T-Lymphocytes / cytology*, immunology |
| Grant Support | |
ID/Acronym/Agency:
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CA42006/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, CD40; 0/Antigens, Differentiation, B-Lymphocyte; 0/Antigens, Surface; 0/Cell Adhesion Molecules; 0/Cytokines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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