Document Detail


Activation of platelets by heparin-induced thrombocytopenia antibodies in the serotonin release assay is not dependent on the presence of heparin.
MedLine Citation:
PMID:  16150048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The serotonin release assay (SRA) tests for antibodies responsible for heparin-induced thrombocytopenia (HIT). By definition, SRA-positive antibodies cause platelet serotonin release in vitro, in the presence of low concentrations of heparin, but not with excess heparin. Many SRA-positive sera activate platelets in the presence of saline without drug, either as a result of residual heparin in the specimen, or because of intrinsic features of the HIT antibodies. The present experiments show that neither exhaustive heparinase treatment, nor chromatographic removal of heparin abrogates the spontaneous platelet activation caused by these HIT antibodies. This is the first study to systematically demonstrate that in vitro activity of HIT antibodies can be independent of heparin. In addition, T-gel chromatography demonstrated differences among fractions of enzyme-linked-immunosorbent assay (ELISA)-positive HIT antibodies within individual specimens. Certain ELISA-positive fractions had SRA activity while others did not, and the SRA activity was not proportional to HIT antibody ELISA titer. These data suggest that antibodies formed as a result of heparin treatment are heterogeneous, and that some can contribute to the pathogenesis of HIT even when heparin is no longer present.
Authors:
M M Prechel; M K McDonald; W P Jeske; H L Messmore; J M Walenga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-09-09
Journal Detail:
Title:  Journal of thrombosis and haemostasis : JTH     Volume:  3     ISSN:  1538-7933     ISO Abbreviation:  J. Thromb. Haemost.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-30     Completed Date:  2005-12-14     Revised Date:  2012-01-10    
Medline Journal Info:
Nlm Unique ID:  101170508     Medline TA:  J Thromb Haemost     Country:  England    
Other Details:
Languages:  eng     Pagination:  2168-75     Citation Subset:  IM    
Affiliation:
Department of Pathology, Loyola University Medical Center, Maywood, IL 60153, USA. mpreche@lumc.edu
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MeSH Terms
Descriptor/Qualifier:
Antibodies / isolation & purification,  physiology*
Carbon Radioisotopes / diagnostic use
Heparin / adverse effects,  immunology*
Heparin Lyase / metabolism
Humans
Immunoglobulin G / isolation & purification
Platelet Activation / drug effects,  immunology*
Serotonin / diagnostic use,  metabolism
Thrombocytopenia / chemically induced,  immunology*
Time Factors
Chemical
Reg. No./Substance:
0/Antibodies; 0/Carbon Radioisotopes; 0/Immunoglobulin G; 50-67-9/Serotonin; 9005-49-6/Heparin; EC 4.2.2.7/Heparin Lyase
Comments/Corrections
Comment In:
J Thromb Haemost. 2011 Dec;9(12):2386-8   [PMID:  21929685 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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