Document Detail


Activation of a plant nucleotide binding-leucine rich repeat disease resistance protein by a modified self protein.
MedLine Citation:
PMID:  22372664     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nucleotide binding-leucine rich repeat (NB-LRR) proteins function as intracellular receptors for the detection of pathogens in both plants and animals. Despite their central role in innate immunity, the molecular mechanisms that govern NB-LRR activation are poorly understood. The Arabidopsis NB-LRR protein RPS5 detects the presence of the Pseudomonas syringae effector protein AvrPphB by monitoring the status of the Arabidopsis protein kinase PBS1. AvrPphB is a cysteine protease that targets PBS1 for cleavage at a single site within the activation loop of PBS1. Using a transient expression system in the plant Nicotiana benthamiana and stable transgenic Arabidopsis plants we found that both PBS1 cleavage products are required to activate RPS5 and can do so in the absence of AvrPphB. We also found, however, that the requirement for cleavage of PBS1 could be bypassed simply by inserting five amino acids at the PBS1 cleavage site, which is located at the apex of the activation loop of PBS1. Activation of RPS5 did not require PBS1 kinase function, and thus RPS5 appears to sense a subtle conformational change in PBS1, rather than cleavage. This finding suggests that NB-LRR proteins may function as fine-tuned sensors of alterations in the structures of effector targets.
Authors:
Brody J DeYoung; Dong Qi; Sang-Hee Kim; Thomas P Burke; Roger W Innes
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-27
Journal Detail:
Title:  Cellular microbiology     Volume:  14     ISSN:  1462-5822     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-15     Completed Date:  2012-10-09     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1071-84     Citation Subset:  IM    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / immunology*,  microbiology*
Arabidopsis Proteins / biosynthesis*,  metabolism*
Bacterial Proteins / metabolism*
Plant Diseases / immunology,  microbiology
Protein-Serine-Threonine Kinases / metabolism*
Pseudomonas syringae / immunology,  pathogenicity*
Tobacco / immunology,  microbiology
Transcriptional Activation*
Grant Support
ID/Acronym/Agency:
GM046451/GM/NIGMS NIH HHS; GM080912-01A1/GM/NIGMS NIH HHS; R01 GM046451/GM/NIGMS NIH HHS; R01 GM046451-18/GM/NIGMS NIH HHS; R01 GM046451-19/GM/NIGMS NIH HHS; R01 GM046451-20/GM/NIGMS NIH HHS; R01 GM046451-21/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Bacterial Proteins; 0/RPS5 protein, Arabidopsis; 0/avrPphB protein, Pseudomonas syringae; EC 2.7.1.-/PBS1 protein, Arabidopsis; EC 2.7.11.1/Protein-Serine-Threonine Kinases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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