Document Detail

Activation of phospholipase A2 by 1,25 (OH)2 vitamin D3 and cell growth in monocytic U937 and Mono Mac 6 cells.
MedLine Citation:
PMID:  7720186     Owner:  NLM     Status:  MEDLINE    
Soluble phospholipase A2 activity was characterized in two human monocytic cell lines, U937 and Mono Mac 6. The enzyme showed an absolute requirement for Ca++, an alkaline pH optimum and Michaelis-Menten kinetics in both cell lines. Differentiation of U937 and Mono Mac 6 cells with 1,25 (OH)2 vitamin D3 (10 nM, 72 h) enhanced PLA2 activity by 82 per cent and 56 per cent, respectively. Furthermore, kinetic experiments revealed that enzyme activity increased within 3 h when cells were brought from the nonproliferative phase of growth to the start of a new cycle of cell proliferation. This initial activation of PLA2 could be inhibited by cycloheximide and actinomycin D, indicating the requirement of gene transcription. Taken together, these results suggest a role of cytosolic, Ca(++)-dependent PLA2 in differentiation and growth of monocytic cells.
F C Aepfelbacher; P C Weber; M Aepfelbacher
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  13     ISSN:  0263-6484     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-05-24     Completed Date:  1995-05-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  19-23     Citation Subset:  IM    
Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Universität München, F.R.G.
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MeSH Terms
Calcitriol / pharmacology*
Calcium / physiology
Cell Differentiation / drug effects
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Enzyme Activation / drug effects
Enzyme Induction / drug effects
Hydrogen-Ion Concentration
Monocytes / drug effects*,  enzymology
Phospholipases A / metabolism*
Phospholipases A2
Protein Biosynthesis / drug effects
Transcription, Genetic / drug effects
Tumor Cells, Cultured
Reg. No./Substance:
32222-06-3/Calcitriol; 50-76-0/Dactinomycin; 66-81-9/Cycloheximide; 7440-70-2/Calcium; EC 3.1.1.-/Phospholipases A; EC A2

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