| Activation of phospholipase A2 by 1,25 (OH)2 vitamin D3 and cell growth in monocytic U937 and Mono Mac 6 cells. | |
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MedLine Citation:
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PMID: 7720186 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Soluble phospholipase A2 activity was characterized in two human monocytic cell lines, U937 and Mono Mac 6. The enzyme showed an absolute requirement for Ca++, an alkaline pH optimum and Michaelis-Menten kinetics in both cell lines. Differentiation of U937 and Mono Mac 6 cells with 1,25 (OH)2 vitamin D3 (10 nM, 72 h) enhanced PLA2 activity by 82 per cent and 56 per cent, respectively. Furthermore, kinetic experiments revealed that enzyme activity increased within 3 h when cells were brought from the nonproliferative phase of growth to the start of a new cycle of cell proliferation. This initial activation of PLA2 could be inhibited by cycloheximide and actinomycin D, indicating the requirement of gene transcription. Taken together, these results suggest a role of cytosolic, Ca(++)-dependent PLA2 in differentiation and growth of monocytic cells. |
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Authors:
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F C Aepfelbacher; P C Weber; M Aepfelbacher |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biochemistry and function Volume: 13 ISSN: 0263-6484 ISO Abbreviation: Cell Biochem. Funct. Publication Date: 1995 Mar |
Date Detail:
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Created Date: 1995-05-24 Completed Date: 1995-05-24 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8305874 Medline TA: Cell Biochem Funct Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 19-23 Citation Subset: IM |
Affiliation:
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Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Universität München, F.R.G. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calcitriol
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pharmacology* Calcium / physiology Cell Differentiation / drug effects Cycloheximide / pharmacology Dactinomycin / pharmacology Enzyme Activation / drug effects Enzyme Induction / drug effects Humans Hydrogen-Ion Concentration Kinetics Monocytes / drug effects*, enzymology Phospholipases A / metabolism* Phospholipases A2 Protein Biosynthesis / drug effects Transcription, Genetic / drug effects Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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32222-06-3/Calcitriol; 50-76-0/Dactinomycin; 66-81-9/Cycloheximide; 7440-70-2/Calcium; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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