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Activation of p34cdc2 kinase by cyclin A.
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MedLine Citation:
PMID:  1826688     Owner:  NLM     Status:  MEDLINE    
Functional clam cyclin A and B proteins have been produced using a baculovirus expression system. Both cyclin A and B can induce meiosis I and meiosis II in Xenopus in the absence of protein synthesis. Half-maximal induction occurs at 50 nM for cyclin A and 250 nM for cyclin B. Addition of 25 nM cyclin A to activated Xenopus egg extracts arrested in the cell cycle by treatment with RNase or emetine activates cdc2 kinase to the normal metaphase level and stimulates one oscillatory cell cycle. High levels of cyclin A cause marked hyperactivation of cdc2 kinase and a stable arrest at the metaphase point in the cell cycle. Kinetic studies demonstrate the concentration of cyclin A added does not affect the 10 min lag period required for kinase activation or the timing of maximal activity, but does control the rate of deactivation of cdc2 kinase during exit from mitosis. In addition, exogenous clam cyclin A inhibits the degradation of both A- and B-type endogenous Xenopus cyclins. These results define a system for investigating the biochemistry and regulation of cdc2 kinase activation by cyclin A.
L M Roy; K I Swenson; D H Walker; B G Gabrielli; R S Li; H Piwnica-Worms; J L Maller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  113     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-05-21     Completed Date:  1991-05-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  507-14     Citation Subset:  IM    
Howard Hughes Medical Institute, University of Colorado School of Medicine, Denver 80262.
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MeSH Terms
CDC2 Protein Kinase / metabolism*
Cell Line
Cyclins / metabolism,  pharmacology*
Cycloheximide / pharmacology
Enzyme Activation
Maturation-Promoting Factor / metabolism
Meiosis / drug effects
Mitosis / drug effects
Protein Biosynthesis
Xenopus laevis
Grant Support
Reg. No./Substance:
0/Cyclins; 66-81-9/Cycloheximide; EC Protein Kinase; EC Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
Journal ID (publisher-id): J. Cell Biol.
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 5 Year: 1991
Volume: 113 Issue: 3
First Page: 507 Last Page: 514
ID: 2288969
Publisher Id: 91201401
PubMed Id: 1826688

Activation of p34cdc2 kinase by cyclin A

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