Document Detail


Activation of ornithine decarboxylase in epithelial cells of rat intestine.
MedLine Citation:
PMID:  7570589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intestinal ornithine decarboxylase (ODC) is strongly induced by dietary amino acid and protein feeding. However, the consequence of this induction is unknown. In this study, we analyzed the relationship between intestinal ODC activity and DNA synthesis in villus and crypt cells of rat intestine. Single amino acid diets and protein diets stimulated ODC activity in villus cells, but not in crypt cells. However a 20% casein diet induced ODC activity and increased the putrescine concentration in villus and crypt cells. Administration of alpha-difluoromethylornithine, a suicide inhibitor of ODC, prevented both an increase in putrescine level and DNA synthesis in the crypt cells. Observations suggested that the induction of ODC is necessary to initiate DNA synthesis in rat intestinal epithelium.
Authors:
F Takahashi; K Miyamoto; H Minami; E Takeda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Tokushima journal of experimental medicine     Volume:  42     ISSN:  0040-8875     ISO Abbreviation:  Tokushima J. Exp. Med.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-11-08     Completed Date:  1995-11-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0417356     Medline TA:  Tokushima J Exp Med     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  11-5     Citation Subset:  IM    
Affiliation:
Department of Food Science, Shizuoka Eiwa College, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
DNA / biosynthesis
Enzyme Activation
Epithelium / enzymology
Intestines / enzymology*
Male
Ornithine Decarboxylase / metabolism*
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
9007-49-2/DNA; EC 4.1.1.17/Ornithine Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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