Document Detail

Activation of normal neutrophils by anti-neutrophil cytoplasm antibodies.
MedLine Citation:
PMID:  1424279     Owner:  NLM     Status:  MEDLINE    
Anti-neutrophil cytoplasm antibodies (ANCA) are markers of systemic vasculitis for which a pathogenetic role has been postulated. We have examined the effect of these autoantibodies on the function of normal human neutrophils in vitro. In the presence of ANCA positive sera luminol-amplified chemiluminescence was significantly increased compared to the values seen in the presence of normal or anti-double stranded DNA positive sera (P < 0.01). Five of six ANCA positive F(ab)2 preparations also produced significant neutrophil activation as demonstrated by the chemiluminescence response. This response was totally abrogated by the addition of neutrophil cytoplasm extract, containing the ANCA antigen. Addition of inhibitors to the chemiluminescence system demonstrated that the chemiluminescence response was inhibited by azide and salicylhydroxamic acid and reduced by histidine, suggesting that the chemiluminescence response was due to activation of myeloperoxidase, with generation of singlet oxygen. The chemotactic response to f-Met-Leu-Phe, a bacterial chemotactic peptide, was significantly augmented in the presence of ANCA. Chemotaxis to zymosan-activated serum and chemokinesis was not affected. Phagocytosis was also unaffected. We propose that neutrophil activation and modulation of neutrophil migration by ANCA may be of pathogenetic significance in systemic vasculitis.
M T Keogan; V L Esnault; A J Green; C M Lockwood; D L Brown
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  90     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1992-12-10     Completed Date:  1992-12-10     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  228-34     Citation Subset:  IM    
Department of Clinical Immunology, Addenbrooke's Hospital, Cambridge, UK.
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MeSH Terms
Antibodies, Antineutrophil Cytoplasmic
Antigen-Antibody Reactions
Autoantibodies / immunology*
Chemiluminescent Measurements
Chemotaxis, Leukocyte
Cytoplasmic Granules / immunology
Immunoglobulin Fab Fragments / immunology
Neutrophils / immunology*
Receptors, Complement 3b / immunology
Respiratory Burst
Reg. No./Substance:
0/Antibodies, Antineutrophil Cytoplasmic; 0/Autoantibodies; 0/Immunoglobulin Fab Fragments; 0/Receptors, Complement 3b

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