Document Detail

Activation of multiple transcription factors and fos and jun gene family expression in cells exposed to a single electric pulse.
MedLine Citation:
PMID:  7589234     Owner:  NLM     Status:  MEDLINE    
We report that exposure of cells to a single electric pulse (250-1250 V/cm) results in the rapid and persistent activation of the DNA binding activities of a number of transcription factors, including AP-1, SP1, AP-2, and NF-kappa B, and the transient expression of select members of the fos and jun gene families. Induction of gene expression occurs primarily at the level of transcription, although c-jun expression also appears to be regulated posttranscriptionally. Interestingly, maximal induction of gene expression is detected at electrical field strengths that do not result in pore formation in the plasma membrane and that do not significantly affect cell viability. Exposure of cells to electric pulses does not result in the activation of HSF1 DNA binding activity, or the induction of hsp70 or p53 protein synthesis, indicating that the induction of fos and jun gene expression is not coincident with protein or DNA damage. The results of these studies suggest that electrical pulses may represent a novel mechanism for inducing the activities of multiple transcription factors and the expression of select members of the fos and jun gene families.
T Pazmany; S P Murphy; S O Gollnick; S P Brooks; T B Tomasi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  221     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-14     Completed Date:  1995-12-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  103-10     Citation Subset:  IM    
Department of Molecular Medicine, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
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MeSH Terms
3T3 Cells / physiology
Base Sequence
CHO Cells / physiology
Cycloheximide / pharmacology
DNA-Binding Proteins / metabolism,  physiology
Electric Stimulation
Gene Expression / physiology*
Genes, fos / physiology*
Genes, jun / physiology*
Lymphoma, B-Cell
Molecular Sequence Data
Signal Transduction / physiology
Transcription Factors / metabolism*
Transcription, Genetic / physiology
Grant Support
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Transcription Factors; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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