Document Detail


Activation of multiple transcription factors and fos and jun gene family expression in cells exposed to a single electric pulse.
MedLine Citation:
PMID:  7589234     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report that exposure of cells to a single electric pulse (250-1250 V/cm) results in the rapid and persistent activation of the DNA binding activities of a number of transcription factors, including AP-1, SP1, AP-2, and NF-kappa B, and the transient expression of select members of the fos and jun gene families. Induction of gene expression occurs primarily at the level of transcription, although c-jun expression also appears to be regulated posttranscriptionally. Interestingly, maximal induction of gene expression is detected at electrical field strengths that do not result in pore formation in the plasma membrane and that do not significantly affect cell viability. Exposure of cells to electric pulses does not result in the activation of HSF1 DNA binding activity, or the induction of hsp70 or p53 protein synthesis, indicating that the induction of fos and jun gene expression is not coincident with protein or DNA damage. The results of these studies suggest that electrical pulses may represent a novel mechanism for inducing the activities of multiple transcription factors and the expression of select members of the fos and jun gene families.
Authors:
T Pazmany; S P Murphy; S O Gollnick; S P Brooks; T B Tomasi
Related Documents :
7724734 - Heterogeneity in c-jun gene expression in normal and malignant cells exposed to either ...
1339344 - Identification of nf-jun, a novel inducible transcription factor that regulates c-jun g...
20566634 - P27 suppresses arsenite-induced hsp27/hsp70 expression through inhibiting jnk2/c-jun- a...
1315324 - Induction of differentiation and c-jun expression in human leukemic cells by okadaic ac...
20627384 - Aberrant microrna expression pattern in myelodysplastic bone marrow cells.
21394934 - Distribution and function of splash, an achaete-scute homolog in the adult olfactory or...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  221     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-14     Completed Date:  1995-12-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  103-10     Citation Subset:  IM    
Affiliation:
Department of Molecular Medicine, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
3T3 Cells / physiology
Animals
Base Sequence
CHO Cells / physiology
Cricetinae
Cycloheximide / pharmacology
DNA-Binding Proteins / metabolism,  physiology
Electric Stimulation
Electroporation
Gene Expression / physiology*
Genes, fos / physiology*
Genes, jun / physiology*
Lymphoma, B-Cell
Mice
Molecular Sequence Data
Rats
Signal Transduction / physiology
Transcription Factors / metabolism*
Transcription, Genetic / physiology
Grant Support
ID/Acronym/Agency:
HD-17013/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Transcription Factors; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Protective effect of cell-permeable ceramide analogs against modeccin, ricin, Pseudomonas toxin, and...
Next Document:  Rat HPC-1/syntaxin 1A and syntaxin 1B interrupt intracellular membrane transport and inhibit secreti...