| Activation of mu- and delta-opioid receptors present on the same nerve terminals depresses transmitter release in the mouse hypogastric ganglion. | |
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MedLine Citation:
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PMID: 1981687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. The inhibitory actions of mu- and delta-opioid receptor agonists on the strong, single fibre synaptic input to neurones contained in the mouse hypogastric ganglion have been examined. 2. The opioid agonists [D-Ala2,NMePhe4,Gly-ol5]enkephalin (DAMGO, 10 nM-10 microM), morphine (10-30 [D-Ser2,Leu5,Thr6]enkephalin (DSLET, 3 nM-1 microM), [D-Pen2,D-Pen5]enkephalin (DPDPE, 10 nM-10 microM), all depressed the single fibre, all-or-nothing, nicotinic, excitatory synaptic potential (e.p.s.p.) recorded in mouse hypogastric ganglion neurones. U50488H (0.3-1 microM) was without effect. 3. The effect of DSLET, but not that of DAMGO, was reversed by the delta-opioid receptor-selective antagonist, ICI 174864 (0.3 microM). Naloxone (0.3 microM) antagonized the effect of both DSLET and DAMGO. 4. The site of action of the mu- and delta-receptor agonists was on the presynaptic terminals, since at the concentrations which depressed the e.p.s.p. these drugs did not affect the resting membrane potential or input resistance of the postganglionic neurone body, nor did they depress the postganglionic, nicotinic response to exogenously applied acetylcholine. 5. Quantal analysis further confirmed the presynaptic site of action; mu- and delta-opioid receptor agonists decreased the mean number of quanta released per stimulus but did not reduce the mean amplitude of the quantal unit. 6. It was concluded that mu- and delta-opioid receptors were located on the same presynaptic nerve terminals since, in the same neurones, mu- and delta-opioid receptor agonists depressed the same single fibre inputs. 7. The potassium channel blockers barium and quinine, at concentrations known to block opioidactivated somatic potassium conductances, reduced slightly but did not abolish the mu- and delta-opioid receptor-mediated inhibition of the e.p.s.p. |
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Authors:
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H Rogers; G Henderson |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of pharmacology Volume: 101 ISSN: 0007-1188 ISO Abbreviation: Br. J. Pharmacol. Publication Date: 1990 Nov |
Date Detail:
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Created Date: 1991-04-22 Completed Date: 1991-04-22 Revised Date: 2010-09-09 |
Medline Journal Info:
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Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 505-12 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Cambridge. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Enkephalin, Ala(2)-MePhe(4)-Gly(5)- Enkephalin, D-Penicillamine (2,5)- Enkephalin, Leucine / analogs & derivatives* Enkephalins / pharmacology Evoked Potentials / drug effects Ganglia / drug effects, physiology Male Mice Mice, Inbred DBA Nerve Endings / drug effects, physiology Neurotransmitter Agents / secretion* Oligopeptides / pharmacology Potassium Channels / drug effects, physiology Receptors, Opioid / drug effects, physiology* Receptors, Opioid, delta Receptors, Opioid, mu |
| Chemical | |
Reg. No./Substance:
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0/Enkephalins; 0/Neurotransmitter Agents; 0/Oligopeptides; 0/Potassium Channels; 0/Receptors, Opioid; 0/Receptors, Opioid, delta; 0/Receptors, Opioid, mu; 100929-53-1/Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; 58822-25-6/Enkephalin, Leucine; 75644-90-5/enkephalin, Ser(2), Leu(5), Thr(6)-; 88373-73-3/Enkephalin, D-Penicillamine (2,5)- |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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